Different routes of bacterial infection induce long-lived TH1 memory cells and short-lived TH17 cells

Marion Pepper; Jonathan L Linehan; Antonio J Pagán; Traci Zell; Thamotharampillai Dileepan; P Patrick Cleary; Marc K Jenkins

doi:10.1038/ni.1826

Different routes of bacterial infection induce long-lived TH1 memory cells and short-lived TH17 cells

Nat Immunol. 2010 Jan;11(1):83-9. doi: 10.1038/ni.1826. Epub 2009 Nov 22.

Authors

Marion Pepper¹, Jonathan L Linehan, Antonio J Pagán, Traci Zell, Thamotharampillai Dileepan, P Patrick Cleary, Marc K Jenkins

Affiliation

¹ Department of Microbiology, Center for Immunology, University of Minnesota Medical School, Minneapolis, Minnesota, USA.

PMID: 19935657
PMCID: PMC2795784
DOI: 10.1038/ni.1826

Abstract

We used a sensitive method based on tetramers of peptide and major histocompatibility complex II (pMHCII) to determine whether CD4(+) memory T cells resemble the T helper type 1 (T(H)1) and interleukin 17 (IL-17)-producing T helper (T(H)17) subsets described in vitro. Intravenous or intranasal infection with Listeria monocytogenes induced pMHCII-specific CD4(+) naive T cells to proliferate and produce effector cells, about 10% of which resembled T(H)1 or T(H)17 cells, respectively. T(H)1 cells were also present among the memory cells that survived 3 months after infection, whereas T(H)17 cells disappeared. The short lifespan of T(H)17 cells was associated with small amounts of the antiapoptotic protein Bcl-2, the IL-15 receptor and the receptor CD27, and little homeostatic proliferation. These results suggest that T(H)1 cells induced by intravenous infection are more efficient at entering the memory pool than are T(H)17 cells induced by intranasal infection.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Amino Acid Sequence
Animals
CD4-Positive T-Lymphocytes / immunology
CD4-Positive T-Lymphocytes / metabolism
CD4-Positive T-Lymphocytes / pathology
Cell Differentiation / immunology
Cell Line
Cell Proliferation
Cell Survival / immunology
Clone Cells / immunology
Clone Cells / pathology
Flow Cytometry
Histocompatibility Antigens Class II / genetics
Histocompatibility Antigens Class II / immunology
Host-Pathogen Interactions / immunology
Immunologic Memory / immunology
Interferon-gamma / metabolism
Interleukin-17 / immunology*
Interleukin-17 / metabolism
Listeria monocytogenes / genetics
Listeria monocytogenes / physiology
Listeriosis / immunology*
Listeriosis / microbiology
Mice
Mice, Inbred C57BL
Mice, Inbred Strains
Proto-Oncogene Proteins c-bcl-2 / metabolism
Receptors, Interleukin-15 / metabolism
T-Lymphocytes, Helper-Inducer / immunology*
T-Lymphocytes, Helper-Inducer / metabolism
T-Lymphocytes, Helper-Inducer / pathology
Th1 Cells / immunology*
Th1 Cells / metabolism
Th1 Cells / pathology
Time Factors
Tumor Necrosis Factor Receptor Superfamily, Member 7 / metabolism

Substances

Histocompatibility Antigens Class II
Interleukin-17
Proto-Oncogene Proteins c-bcl-2
Receptors, Interleukin-15
Tumor Necrosis Factor Receptor Superfamily, Member 7
Interferon-gamma

Abstract

Publication types

MeSH terms

Substances

Grants and funding