Abstract
Stress-responsive genes play critical roles in many biological functions that includes apoptosis, survival, differentiation and regeneration. We have identified a novel stress-responsive gene called BRE which interacts with TNF-receptor-1 and blocks the apoptotic effect of TNF-alpha. BRE enhances tumor growth in vivo and is up-regulated in hepatocellular and esophageal carcinomas. BRE also regulates the ubiquitination of the DNA repair complex BRCC, and the synthesis of steroid hormones. Here, we examined BRE-mRNA in cells after treatments with UV and ionizing radiation (IR). UV and IR treatment alone suppressed BRE-mRNA levels by more than 90% at 24 h, while hydroxyurea, fluorodeoxyuridine, aphidicolin, known inhibitors of S-phase DNA synthesis, had no significant effect. BRE protein expression was unaltered in cells treated with TNF-alpha, Interleukin-1 and Dexamethasone, while a threefold increase was observed following chorionic gonadotropin exposure. Although BRE plays a regulatory role in many different pathways, yet its expression is apparently under very stringent control.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aphidicolin / pharmacology
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Biological Factors / pharmacology*
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Cadaverine / analogs & derivatives
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Cadaverine / pharmacology
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Cell Line
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Chorionic Gonadotropin / pharmacology
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DNA Ligase ATP
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DNA Ligases / genetics
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DNA Ligases / metabolism
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Dexamethasone / pharmacology
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Dimethyl Sulfoxide / pharmacology
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Fluorodeoxyuridylate / pharmacology
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Gene Expression Regulation / drug effects*
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Gene Expression Regulation / radiation effects
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Humans
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Hydroxyurea / pharmacology
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Interleukin-1 / pharmacology
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / metabolism
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Promyelocytic Leukemia Protein
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Radiation, Ionizing
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Signal Transduction / drug effects*
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Signal Transduction / radiation effects
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Stress, Physiological / drug effects*
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Stress, Physiological / genetics*
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Stress, Physiological / radiation effects
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Tumor Necrosis Factor-alpha / pharmacology
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Tumor Suppressor Proteins / genetics
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Tumor Suppressor Proteins / metabolism
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Ultraviolet Rays
Substances
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BABAM2 protein, human
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Biological Factors
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Chorionic Gonadotropin
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Interleukin-1
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Nerve Tissue Proteins
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Nuclear Proteins
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Promyelocytic Leukemia Protein
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RNA, Messenger
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Transcription Factors
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Tumor Necrosis Factor-alpha
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Tumor Suppressor Proteins
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Fluorodeoxyuridylate
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PML protein, human
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Aphidicolin
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Dexamethasone
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DNA Ligases
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DNA Ligase ATP
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monodansylcadaverine
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Cadaverine
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Hydroxyurea
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Dimethyl Sulfoxide