No association between EGF +61 A/G polymorphism and increased risk of glioma

Int J Biol Markers. 2009 Apr-Jun;24(2):77-82. doi: 10.1177/172460080902400203.

Abstract

A single nucleotide polymorphism (SNP) of the epidermal growth factor (EGF) gene +61 A/G in the 5'-untranslated region has been reported to be associated with susceptibility to glioma. A case-control study (168 glioma patients and 194 normal controls) was conducted to elucidate its possible association with the risk of glioma in the Chinese population. Polymerase chain reaction-restriction fragment length polymorphism assay was used to analyze the EGF genotypes. The genotyping results were further confirmed by direct sequencing. The EGF +61A and +61G allele frequencies in the glioma group were 32.1% and 67.9%, respectively, while they were 30.4% and 69.6% in the healthy controls. Furthermore, the frequency of the A/A, A/G and G/G genotypes in glioma patients was 8.9%, 46.4%, and 44.7%, respectively, and 8.3%, 44.3%, and 47.4% in controls. There was no significant difference between patients and healthy controls. The EGF +61 A/G and +61 G/G genotypes were not significantly associated with risk of glioma compared with the A/A genotype. In addition, no significant association was observed between EGF polymorphism and different histological grades of glioma. These results indicate that the EGF +61 A/G polymorphism is not associated with susceptibility to glioma in the Chinese population. In addition, a literature review revealed a significantly higher rate of the A/A genotype in Caucasian compared with East Asian subjects. Such differences in genotype distribution between Caucasian and Asian people should be taken into account in future studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions
  • Adult
  • Aged
  • Brain Neoplasms / genetics*
  • Case-Control Studies
  • Epidermal Growth Factor / genetics*
  • Female
  • Genetic Predisposition to Disease*
  • Glioma / diagnosis
  • Glioma / genetics*
  • Humans
  • Male
  • Middle Aged
  • Polymorphism, Genetic*
  • Risk
  • Sequence Analysis, DNA

Substances

  • 5' Untranslated Regions
  • Epidermal Growth Factor