Comprehensive expression analysis of all Wnt genes and their major secreted antagonists during mouse limb development and cartilage differentiation

Florian Witte; Janine Dokas; Franziska Neuendorf; Stefan Mundlos; Sigmar Stricker

doi:10.1016/j.gep.2008.12.009

Comprehensive expression analysis of all Wnt genes and their major secreted antagonists during mouse limb development and cartilage differentiation

Gene Expr Patterns. 2009 Apr;9(4):215-23. doi: 10.1016/j.gep.2008.12.009. Epub 2009 Jan 13.

Authors

Florian Witte¹, Janine Dokas, Franziska Neuendorf, Stefan Mundlos, Sigmar Stricker

Affiliation

¹ Max Planck Institute for Molecular Genetics, Ihnestr. 73, D-14195 Berlin, Germany.

PMID: 19185060
DOI: 10.1016/j.gep.2008.12.009

Abstract

Wnt signalling plays important roles in patterning and outgrowth of the vertebrate limb. Different mutations in Wnt genes, their antagonists or (co-)receptors result in patterning and outgrowth defects as well as chondrocyte and bone phenotypes in mouse and human. Understanding Wnt activity during mouse limb development and chondrogenesis requires a temporal and spatial overview of Wnt signalling key factor expression. Here we present a comparative expression analysis of all 19 Wnt genes and their major secreted antagonists of the Dickkopf (Dkk), Wisp and the secreted frizzled related protein (Sfrp) families during mouse limb development. Our study reveals new domains of expression for Wnt2, Wnt2b, Wnt5b, Wnt6, Wnt7b, Wnt9a, Wnt10a, Wnt10b, Wnt11 and Wnt16, in the limb. We also identified novel expression domains for the Wnt antagonists Sfrp1, Sfrp3, Sfrp5, Wisp1 as well as Dkk2 and Dkk3. We provide a full expression pattern for Wif1 in limb development, for which no limb expression had been documented so far.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing
Animals
CCN Intercellular Signaling Proteins
Cartilage / embryology
Cartilage / metabolism*
Chondrocytes / metabolism
Ectoderm / metabolism
Embryo, Mammalian / embryology
Embryo, Mammalian / metabolism
Extracellular Matrix Proteins / genetics
Forelimb / embryology
Forelimb / metabolism*
Gene Expression Profiling*
Gene Expression Regulation, Developmental*
Glycoproteins / genetics
In Situ Hybridization
Intercellular Signaling Peptides and Proteins / genetics
Intracellular Signaling Peptides and Proteins
Membrane Proteins / genetics
Mesoderm / metabolism
Mice / embryology
Mice / genetics*
Oncogene Proteins / genetics
Proto-Oncogene Proteins
Time Factors
Wnt Proteins / genetics*
Wnt2 Protein

Substances

Adaptor Proteins, Signal Transducing
CCN Intercellular Signaling Proteins
CCN4 protein, mouse
Dkk2 protein, mouse
Dkk3 protein, mouse
Extracellular Matrix Proteins
Glycoproteins
Intercellular Signaling Peptides and Proteins
Intracellular Signaling Peptides and Proteins
Membrane Proteins
Oncogene Proteins
Proto-Oncogene Proteins
Sfrp1 protein, mouse
Sfrp5 protein, mouse
WD repeat containing planar cell polarity effector
Wif1 protein, mouse
Wnt Proteins
Wnt2 Protein