[Expression of galanin and galanin receptors in neurogenesis regions of adult mouse brain and effect of galanin on the neural stem cell's differentiation]

Fen Zi Xi Bao Sheng Wu Xue Bao. 2008 Oct;41(5):359-66.
[Article in Chinese]

Abstract

The neuropeptid galanin is widely expressed in the central nervous system and has a diverse range of physiological effects including food intaking, arousal/sleep, nociception and reproduction. In this study, expression of galanin and galanin receptors (GalR1 and GalR2) mRNA were identified not only in the neurogenisis regions including subventricular zone (SVZ), rostral migratory stream (RMS) and dentate gyrus (DG) of adult mice but also in the SVZ-derived neural stem cell (NSC) culture. Here, we also showed that the addition of galanin and GalR2-specific agonist Gal2-11 to wild-type or GALKO NSCs under differentiation condition significantly promote the neuritogenesis and increase the length of neurites on the betaIII-tubulin positive cells. This effect could be reduced by treatment of the galanin antagonist M35. These results indicate that galanin and its receptors might regulate neurite extension in differentiating neural stem cells and even participate in the development of the nervous system.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Brain / cytology
  • Brain / metabolism*
  • Cell Differentiation / drug effects*
  • Cell Differentiation / genetics
  • Cells, Cultured
  • Galanin / metabolism*
  • Galanin / pharmacology
  • Galanin / physiology*
  • Immunohistochemistry
  • In Situ Hybridization
  • Mice
  • Mice, Inbred C57BL
  • Neurogenesis / genetics
  • Neurogenesis / physiology*
  • Receptors, Galanin / metabolism*
  • Receptors, Galanin / physiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stem Cells / cytology
  • Stem Cells / drug effects*
  • Stem Cells / metabolism

Substances

  • Receptors, Galanin
  • Galanin