Isolation of an additional member of the fibroblast growth factor receptor family, FGFR-3

Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1095-9. doi: 10.1073/pnas.88.4.1095.

Abstract

The fibroblast growth factors are a family of polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing. Fibroblast growth factor receptors (FGFRs) have previously been identified in chicken, mouse, and human and have been shown to contain an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. We have isolated a human cDNA for another tyrosine kinase receptor that is highly homologous to the previously described FGFR. Expression of this receptor cDNA in COS cells directs the expression of a 125-kDa glycoprotein. We demonstrate that this cDNA encodes a biologically active receptor by showing that human acidic and basic fibroblast growth factors activate this receptor as measured by 45Ca2+ efflux assays. These data establish the existence of an additional member of the FGFR family that we have named FGFR-3.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Calcium / metabolism
  • Cell Line
  • Cloning, Molecular
  • Fibroblast Growth Factors / metabolism
  • Gene Library
  • Humans
  • Molecular Sequence Data
  • Multigene Family*
  • Oligonucleotide Probes
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / isolation & purification
  • Receptors, Fibroblast Growth Factor
  • Transfection

Substances

  • Oligonucleotide Probes
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Fibroblast Growth Factor
  • Fibroblast Growth Factors
  • Calcium

Associated data

  • GENBANK/M58051