A novel PCR-RFLP assay for the detection of the single nucleotide polymorphism at position +1440 in the human CXCR2 gene

Aline C Viana; Yeon J Kim; Joni A Cirelli; Silvana R P Orrico; Karen C Curtis; Veridiana S P Cano; Sandro R Valentini; Raquel Mantuaneli Scarel-Caminaga

doi:10.1007/s10528-007-9111-0

A novel PCR-RFLP assay for the detection of the single nucleotide polymorphism at position +1440 in the human CXCR2 gene

Biochem Genet. 2007 Oct;45(9-10):737-41. doi: 10.1007/s10528-007-9111-0. Epub 2007 Oct 16.

Authors

Aline C Viana¹, Yeon J Kim, Joni A Cirelli, Silvana R P Orrico, Karen C Curtis, Veridiana S P Cano, Sandro R Valentini, Raquel Mantuaneli Scarel-Caminaga

Affiliation

¹ Department of Diagnosis and Surgery, School of Dentistry, UNESP - São Paulo State University, R. Humaitá, 1680 Centro, Araraquara, Sao Paulo, 14801-903, Brazil.

PMID: 17939034
DOI: 10.1007/s10528-007-9111-0

Abstract

We designed a novel PCR-RFLP assay to genotype for the CXCR2 +1440 (G/A) single nucleotide polymorphism, which provides a simple, low-cost, practical, and reproducible method. Allele frequencies in healthy Brazilian individuals were found to be 0.65% for allele A and 0.35% for allele G.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Amplified Fragment Length Polymorphism Analysis / methods
Base Sequence
Brazil
Case-Control Studies
DNA Primers / genetics
Gene Frequency
Humans
Periodontal Diseases / genetics
Periodontal Diseases / immunology
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide*
Receptors, Interleukin-8B / genetics*

Substances

DNA Primers
Receptors, Interleukin-8B