Strong induction of the Tis11B gene in myogenic differentiation

Melanie Busse; Max Schwarzburger; Felicitas Berger; Christine Hacker; Barbara Munz

doi:10.1016/j.ejcb.2007.07.005

Strong induction of the Tis11B gene in myogenic differentiation

Eur J Cell Biol. 2008 Jan;87(1):31-8. doi: 10.1016/j.ejcb.2007.07.005. Epub 2007 Sep 24.

Authors

Melanie Busse¹, Max Schwarzburger, Felicitas Berger, Christine Hacker, Barbara Munz

Affiliation

¹ Institute of Physiology, Charité - University Medicine Berlin, Arnimallee 22, D-14195 Berlin, Germany.

PMID: 17889962
DOI: 10.1016/j.ejcb.2007.07.005

Abstract

TIS11B is a zinc-finger protein of the tristetraprolin (TTP) family. Using cDNA microarray analysis, we could identify the Tis11B gene based on its differential expression in myogenesis. Here, we demonstrate that expression of the Tis11B gene is strongly induced during differentiation of the murine myoblast cell line C2C12. By contrast, expression of Ttp itself was not induced in myogenesis. Pretreatment of the cells with the translation inhibitor cycloheximide demonstrated that Tis11B was a primary response gene in this process. In addition, pretreatment with the transcription inhibitor actinomycin D demonstrated that gene expression was regulated at the transcriptional level. Since specific inhibitors of p38 MAP kinase completely blocked Tis11B induction, we conclude that expression of the Tis11B gene is regulated at least in part by this signaling pathway which plays a central role in myogenesis. Induction of Tis11B expression was also observed in primary myoblasts isolated from two different mouse strains, indicating physiological relevance of our results. In addition, TIS11B might also be an important player during myogenic differentiation and regeneration in vivo, as we detected a marked decrease in expression in several muscle tissues of the dystrophic mdx mouse, a model for continuous muscle degeneration and regeneration. These data suggest that TIS11B is an important regulator of myogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Butyrate Response Factor 1
Cell Differentiation / drug effects
Cell Differentiation / physiology*
Cell Line
Cycloheximide / pharmacology
Dactinomycin / pharmacology
Disease Models, Animal
Gene Expression Profiling
Gene Expression Regulation / drug effects
Gene Expression Regulation / physiology*
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred mdx
Muscle Development / drug effects
Muscle Development / physiology*
Muscular Dystrophies / genetics
Muscular Dystrophies / metabolism
Muscular Dystrophy, Animal / genetics
Muscular Dystrophy, Animal / metabolism
Myoblasts / cytology
Myoblasts / metabolism*
Nuclear Proteins / biosynthesis*
Nuclear Proteins / genetics
Oligonucleotide Array Sequence Analysis
Protein Kinase Inhibitors / pharmacology
Protein Synthesis Inhibitors / pharmacology
RNA-Binding Proteins / biosynthesis*
RNA-Binding Proteins / genetics
Regeneration / drug effects
Regeneration / physiology*
Signal Transduction / drug effects
Signal Transduction / physiology
Transcription, Genetic / drug effects
Transcription, Genetic / physiology
p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors
p38 Mitogen-Activated Protein Kinases / metabolism

Substances

Butyrate Response Factor 1
Nuclear Proteins
Protein Kinase Inhibitors
Protein Synthesis Inhibitors
RNA-Binding Proteins
Zfp36l1 protein, mouse
Dactinomycin
Cycloheximide
p38 Mitogen-Activated Protein Kinases