Abstract
NIPP1 is a ubiquitously expressed nuclear protein that represses the transcription of targeted genes. Here we show that the transcriptional repression by NIPP1 is alleviated by the RNAi-mediated knockdown of EED and EZH2, two core components of the Polycomb Repressive Complex 2 (PRC2), and by the overexpression of a catalytically dead mutant of the histone methyltransferase EZH2. NIPP1 is present in a complex with EED and EZH2 in vivo and has distinct binding sites for these proteins. These data disclose an essential role for the PRC2 complex in the transcriptional repression by NIPP1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding Sites / genetics
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Cell Line, Tumor
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Endoribonucleases / genetics
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Endoribonucleases / metabolism*
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Enhancer of Zeste Homolog 2 Protein
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Humans
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Multiprotein Complexes / genetics
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Multiprotein Complexes / metabolism*
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Phosphoprotein Phosphatases / genetics
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Phosphoprotein Phosphatases / metabolism*
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Polycomb Repressive Complex 2
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Polycomb-Group Proteins
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Protein Binding / genetics
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / metabolism*
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic / physiology*
Substances
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DNA-Binding Proteins
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EED protein, human
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Multiprotein Complexes
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Polycomb-Group Proteins
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RNA-Binding Proteins
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Repressor Proteins
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Transcription Factors
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EZH2 protein, human
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Enhancer of Zeste Homolog 2 Protein
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Polycomb Repressive Complex 2
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Endoribonucleases
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Phosphoprotein Phosphatases
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PPP1R8 protein, human