Abstract
Recent research suggests that the D2-40 monoclonal antibody recognizes the 40,000 Da O-linked sialoglycoprotein podoplanin. Podoplanin not only is highly expressed in lymphatic endothelium but also in other cell types, including sebaceous carcinoma cells. Using the D2-40 antibody, our purpose was to evaluate expression of podoplanin in sebaceous glands of normal skin. Twenty-four formalin-fixed, paraffin-embedded normal skin specimens (10 from scalp and 14 from cheeks) were immunostained using the D2-40 mouse monoclonal antibody. Strong immunostaining with D2-40 antibody was observed at the periphery of sebaceous glands and in skin lymphatic endothelium of all specimens, demonstrating that podoplanin is expressed in sebaceous glands of normal skin.
MeSH terms
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Adenoma / metabolism
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Adenoma / pathology
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Adult
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Aged
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Aged, 80 and over
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Antibodies, Monoclonal / immunology
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Antibodies, Monoclonal / metabolism*
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Antibodies, Monoclonal, Murine-Derived
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Biomarkers, Tumor / metabolism
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Carcinoma / metabolism
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Carcinoma / pathology
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Endothelium, Lymphatic / cytology
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Endothelium, Lymphatic / metabolism*
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Female
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Humans
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Male
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Membrane Glycoproteins / immunology
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Membrane Glycoproteins / metabolism*
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Middle Aged
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Sebaceous Gland Neoplasms / metabolism
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Sebaceous Gland Neoplasms / pathology
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Sebaceous Glands / metabolism*
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Sebaceous Glands / pathology
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Skin / cytology
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Skin / metabolism*
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Skin / pathology
Substances
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Antibodies, Monoclonal
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Antibodies, Monoclonal, Murine-Derived
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Biomarkers, Tumor
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Membrane Glycoproteins
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PDPN protein, human
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monoclonal antibody D2-40