Sca-1 and Thy-1 accelerate neuron-like differentiation in bone marrow stromal cells

Kobe J Med Sci. 2006;52(5):119-25.

Abstract

Bone marrow stromal cells taken from EGFP transgenic mice were sorted by magnetic beads with surface markers for Sca-1 and Thy-1. The cells were then co-cultured on organotypic hippocampal slice or with neuronal cell feeder in dish. On hippocampus, both Sca-1 and Thy-1 positive cells showed 4- 8 folds higher potential to show neuron-like morphology than negative cells. In dish, negative cells fewly survived but each positive cells survived and showed neuron-like differentiation. In both culture condition, retinoic acid supplement accelerate differentiation. Differentiated Sca-1 and Thy-1 positive cells were immunohistochemically GFAP- and NeuN-negative but nestin-, neurofilament- and NSE-positive. Neuron-like differentiation of bone marrow cells can be enhanced by selection using cell surface proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Ly / biosynthesis
  • Antigens, Ly / genetics
  • Antigens, Ly / physiology*
  • Bone Marrow Cells / cytology*
  • Bone Marrow Cells / metabolism
  • Cell Differentiation / physiology*
  • Cells, Cultured
  • Coculture Techniques
  • Genes, Reporter
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / genetics
  • Membrane Proteins / physiology*
  • Mice
  • Neurons / cytology*
  • Neurons / metabolism
  • Stromal Cells / cytology
  • Stromal Cells / metabolism
  • Thy-1 Antigens / biosynthesis
  • Thy-1 Antigens / genetics
  • Thy-1 Antigens / physiology*

Substances

  • Antigens, Ly
  • Ly6a protein, mouse
  • Membrane Proteins
  • Thy-1 Antigens