Abstract
Background:
There are few biological markers, which strictly show the severity of congestive heart failure (CHF).
Methods and results:
Lymphocyte G-protein coupled receptor kinase (GRK) mRNA expression was measured by quantitative reverse transcription-polymerase chain reaction in 15 CHF patients: 5 patients classified as New York Heart Association class-II treated with angiotensin converting enzyme inhibitor (ACEI) (IIA), 5 patients in class-II without ACEI (IIC), and 5 patients in class-III treated with ACEI (IIIA). GRK mRNA level in IIIA was significantly higher than those in IIA (p<0.05). GRK mRNA level in IIA were significantly lower than those in IIC (p<0.05).
Conclusions:
The expression level of lymphocyte GRK might show the severity of CHF, and ACEI treatment could reduce the level of GRK in CHF patients.
MeSH terms
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Adult
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Aged
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Angiotensin-Converting Enzyme Inhibitors / pharmacology*
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Angiotensin-Converting Enzyme Inhibitors / therapeutic use
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Biomarkers / blood
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Female
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G-Protein-Coupled Receptor Kinase 1 / analysis
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G-Protein-Coupled Receptor Kinase 1 / genetics*
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G-Protein-Coupled Receptor Kinase 1 / metabolism
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Gene Expression Regulation, Enzymologic / drug effects*
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Heart Failure / drug therapy
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Heart Failure / enzymology*
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Heart Failure / genetics*
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Humans
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Lymphocytes / chemistry
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Lymphocytes / enzymology
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Male
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Middle Aged
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Receptors, Adrenergic, beta / drug effects
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Receptors, Adrenergic, beta / physiology
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Reverse Transcriptase Polymerase Chain Reaction
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Severity of Illness Index
Substances
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Angiotensin-Converting Enzyme Inhibitors
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Biomarkers
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RNA, Messenger
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Receptors, Adrenergic, beta
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G-Protein-Coupled Receptor Kinase 1