Human class-I restricted T cell associated molecule is highly expressed in the cerebellum and is a marker for activated NKT and CD8+ T lymphocytes

J Neuroimmunol. 2006 Feb;171(1-2):145-55. doi: 10.1016/j.jneuroim.2005.09.017. Epub 2005 Nov 18.

Abstract

We have previously reported the characterization of a novel immunoglobulin supergene family member, designated class-I MHC-restricted T cell associated molecule (CRTAM). Here we further characterize human CRTAM and find that it is highly expressed in the cerebellum, notably in Purkinje neurons. We identify CRTAM as a new member of the nectin-like (Necls) family and find significant expression of Necl-2 (IGSF4), a protein known to bind CRTAM and another member of the nectin superfamily, in the cerebellum. These findings suggest that CRTAM/Necl-2 binding may contribute to neuronal interactions. We also show that, in the immune system, CRTAM expression is restricted to activated class-I MHC-restricted T cells, including NKT and CD8 T cells. CRTAM represents one of the most highly expressed surface markers of activated human CD8 T cells and NKT cells, suggesting it may have diagnostic uses in various human viral and autoimmune diseases.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Western / methods
  • CD8-Positive T-Lymphocytes / metabolism*
  • Cell Adhesion Molecule-1
  • Cell Adhesion Molecules
  • Cerebellum / cytology
  • Cerebellum / metabolism*
  • Dose-Response Relationship, Drug
  • Enzyme Activation / physiology
  • Female
  • Flow Cytometry / methods
  • Gene Expression Regulation, Enzymologic / physiology*
  • Humans
  • Immunoglobulins / metabolism*
  • Immunohistochemistry / methods
  • Ionomycin / pharmacology
  • Leukocytes, Mononuclear / metabolism
  • Lymphocyte Activation / physiology*
  • Membrane Proteins / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Oligonucleotide Array Sequence Analysis / methods
  • Organic Anion Transport Protein 1 / metabolism*
  • Phylogeny
  • Purkinje Cells / metabolism
  • RNA, Messenger / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Sequence Alignment / methods
  • Time Factors
  • Tumor Suppressor Proteins / metabolism

Substances

  • CADM1 protein, human
  • Cell Adhesion Molecule-1
  • Cell Adhesion Molecules
  • Immunoglobulins
  • Membrane Proteins
  • Organic Anion Transport Protein 1
  • RNA, Messenger
  • Slc22a6 protein, mouse
  • Tumor Suppressor Proteins
  • class-I restricted T cell-associated molecule
  • Ionomycin