Abstract
We have previously reported the characterization of a novel immunoglobulin supergene family member, designated class-I MHC-restricted T cell associated molecule (CRTAM). Here we further characterize human CRTAM and find that it is highly expressed in the cerebellum, notably in Purkinje neurons. We identify CRTAM as a new member of the nectin-like (Necls) family and find significant expression of Necl-2 (IGSF4), a protein known to bind CRTAM and another member of the nectin superfamily, in the cerebellum. These findings suggest that CRTAM/Necl-2 binding may contribute to neuronal interactions. We also show that, in the immune system, CRTAM expression is restricted to activated class-I MHC-restricted T cells, including NKT and CD8 T cells. CRTAM represents one of the most highly expressed surface markers of activated human CD8 T cells and NKT cells, suggesting it may have diagnostic uses in various human viral and autoimmune diseases.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Blotting, Western / methods
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CD8-Positive T-Lymphocytes / metabolism*
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Cell Adhesion Molecule-1
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Cell Adhesion Molecules
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Cerebellum / cytology
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Cerebellum / metabolism*
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Dose-Response Relationship, Drug
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Enzyme Activation / physiology
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Female
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Flow Cytometry / methods
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Gene Expression Regulation, Enzymologic / physiology*
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Humans
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Immunoglobulins / metabolism*
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Immunohistochemistry / methods
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Ionomycin / pharmacology
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Leukocytes, Mononuclear / metabolism
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Lymphocyte Activation / physiology*
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Membrane Proteins / metabolism
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Mice
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Mice, Inbred C57BL
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Oligonucleotide Array Sequence Analysis / methods
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Organic Anion Transport Protein 1 / metabolism*
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Phylogeny
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Purkinje Cells / metabolism
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RNA, Messenger / biosynthesis
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Sequence Alignment / methods
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Time Factors
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Tumor Suppressor Proteins / metabolism
Substances
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CADM1 protein, human
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Cell Adhesion Molecule-1
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Cell Adhesion Molecules
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Immunoglobulins
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Membrane Proteins
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Organic Anion Transport Protein 1
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RNA, Messenger
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Slc22a6 protein, mouse
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Tumor Suppressor Proteins
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class-I restricted T cell-associated molecule
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Ionomycin