Abstract
Saitohin is a gene unique to humans and their closest relatives, the function of which is not yet known. Saitohin contains a single polymorphism (Q7R), and its Q and R alleles belong to the H1 and H2 tau haplotype, respectively. The Saitohin Q allele confers susceptibility to several neurodegenerative diseases. To get a handle on Saitohin function, we used it as a bait in a yeast two-hybrid screen. By this assay and subsequent co-immunoprecipitation and glutathione S-transferase pull-down assays, we discovered and confirmed that Saitohin interacts with peroxiredoxin 6, a unique member of that family that is bifunctional and the levels of which increase in Pick disease. The strength of the interaction appeared to be allele-specific, giving the first distinction between the two forms of Saitohin.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Alleles
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Animals
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Base Sequence
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COS Cells
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Cell Line
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Chlorocebus aethiops
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DNA / genetics
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Haplotypes
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Heredodegenerative Disorders, Nervous System / etiology
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Heredodegenerative Disorders, Nervous System / genetics*
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Heredodegenerative Disorders, Nervous System / metabolism*
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Humans
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Peroxidases / genetics*
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Peroxidases / metabolism*
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Peroxiredoxin VI
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Peroxiredoxins
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Pick Disease of the Brain / etiology
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Pick Disease of the Brain / genetics
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Pick Disease of the Brain / metabolism
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RNA Splicing
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Two-Hybrid System Techniques
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tau Proteins / genetics*
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tau Proteins / metabolism*
Substances
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Recombinant Fusion Proteins
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STH protein, human
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tau Proteins
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DNA
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Peroxidases
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PRDX6 protein, human
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Peroxiredoxin VI
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Peroxiredoxins