Spindlin1, a novel nuclear protein with a role in the transformation of NIH3T3 cells

Yanhong Gao; Wen Yue; Peng Zhang; Li Li; Xiaoyan Xie; Hongfeng Yuan; Lin Chen; Daqing Liu; Fang Yan; Xuetao Pei

doi:10.1016/j.bbrc.2005.07.087

Spindlin1, a novel nuclear protein with a role in the transformation of NIH3T3 cells

Biochem Biophys Res Commun. 2005 Sep 23;335(2):343-50. doi: 10.1016/j.bbrc.2005.07.087.

Authors

Yanhong Gao¹, Wen Yue, Peng Zhang, Li Li, Xiaoyan Xie, Hongfeng Yuan, Lin Chen, Daqing Liu, Fang Yan, Xuetao Pei

Affiliation

¹ Laboratory of Stem Cell Biology, Beijing Institute of Transfusion Medicine, 27 Taiping Road, Beijing 100850, PR China.

PMID: 16098913
DOI: 10.1016/j.bbrc.2005.07.087

Abstract

spindlin1, a novel human gene recently isolated by our laboratory, is highly homologous to mouse spindlin gene. In this study, we cloned cDNA full-length of this novel gene and send it to GenBank database as spindlin1 (Homo sapiens spindlin1) with Accession No. AF317228. In order to investigate the function of spindlin1, we studied further the subcellular localization of Spindlin1 protein and the effects of spindlin1 overexpression in NIH3T3 cells. The results showed that the fusion protein pEGFP-N1-spindlin1 was located in the nucleus and the C-terminal is correlated with nuclear localization of Spindlin1 protein. NIH3T3 cells which could stably express spindlin1 as a result of RT-PCR analysis compared with the control cells displayed a complete morphological change; made cell growth faster; and increased the percentage of cells in G2/M and S phase. Furthermore, overexpressed spindlin1 cells formed colonies in soft agar in vitro and formed tumors in nude mice. Our findings provide direct evidence that spindlin1 gene may contribute to tumorigenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agar / chemistry
Animals
Base Sequence
Blotting, Western
COS Cells
Cell Cycle
Cell Cycle Proteins / biosynthesis*
Cell Cycle Proteins / physiology*
Cell Division
Cell Line, Tumor
Cell Nucleus / metabolism
Cell Proliferation
Cloning, Molecular
DNA, Complementary / metabolism
Electrophoresis, Polyacrylamide Gel
Exons
Expressed Sequence Tags
Female
Flow Cytometry
G2 Phase
Gene Expression Profiling
Green Fluorescent Proteins / metabolism
Humans
Introns
Mice
Mice, Nude
Microscopy, Fluorescence
Microtubule-Associated Proteins / biosynthesis*
Microtubule-Associated Proteins / physiology*
Molecular Sequence Data
NIH 3T3 Cells
Open Reading Frames
Ovarian Neoplasms / pathology
Phosphoproteins / biosynthesis*
Phosphoproteins / physiology*
Plasmids / metabolism
Polymerase Chain Reaction
Protein Structure, Tertiary
Reverse Transcriptase Polymerase Chain Reaction
S Phase
Transfection

Substances

Cell Cycle Proteins
DNA, Complementary
Microtubule-Associated Proteins
Phosphoproteins
spindlin
Green Fluorescent Proteins
Agar

Associated data

GENBANK/AF317228