Gas2, a growth arrest-specific protein, is a component of the microfilament network system

J Cell Biol. 1992 Jun;117(6):1251-61. doi: 10.1083/jcb.117.6.1251.

Abstract

In this report we analyze the protein product of a growth arrest-specific gene, gas2, by means of an affinity-purified antibody raised against the protein produced in bacteria. The regulation of Gas2 biosynthesis reflects the pattern of mRNA expression (Schneider, C., R. King, and L. Philipson. 1988. Cell. 54:787-793): its relative level is tightly associated with growth arrest. Gas2 seems to be regulated also at the posttranslational level via a phosphorylation mechanism. Gas2 is well conserved during the evolution with the same apparent molecular mass (36 kD) between mouse and human. We also demonstrate that Gas2 is a component of the microfilament system. It colocalizes with actin fiber, at the cell border and also along the stress fiber, in growth-arrested NIH 3T3 cells. The pattern of distribution, detected in arrested cells, can also be observed in growing cells when they are microinjected with the purified GST-Gas2 protein. In none of the analyzed oncogene-transformed NIH 3T3 cell lines was Gas2 expression induced under serum starvation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Blotting, Western
  • Cell Division
  • Cell Transformation, Neoplastic
  • Escherichia coli / genetics
  • G1 Phase
  • Glutathione Transferase / genetics
  • Glutathione Transferase / metabolism
  • Growth Inhibitors / genetics
  • Growth Inhibitors / metabolism*
  • Humans
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mice
  • Microfilament Proteins / genetics
  • Microfilament Proteins / metabolism*
  • Microinjections
  • Microscopy, Fluorescence
  • Precipitin Tests
  • Protein Processing, Post-Translational
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Resting Phase, Cell Cycle

Substances

  • GAS2L1 protein, human
  • Growth Inhibitors
  • Membrane Proteins
  • Microfilament Proteins
  • Recombinant Fusion Proteins
  • growth inhibitory proteins
  • Glutathione Transferase