Diagnostic usefulness of aberrant CD22 expression in differentiating neoplastic cells of B-Cell chronic lymphoproliferative disorders from admixed benign B cells in four-color multiparameter flow cytometry

Am J Clin Pathol. 2005 Jun;123(6):826-32. doi: 10.1309/KPXN-VR7X-4AME-NBBE.

Abstract

The diagnosis of B-cell chronic lymphoproliferative disorders is a great challenge when made in a background of polyclonal B cells. We studied the diagnostic usefulness of aberrant CD22 expression for differentiating neoplastic from benign B cells by 4-color flow cytometry. Of 56 cases of B-cell chronic lymphoproliferative disorders, we found that neoplastic cells showed aberrant CD22 expression in 39 (70%) of 56 cases, including chronic lymphocytic leukemia, mantle cell lymphoma, marginal zone lymphoma, hairy cell leukemia, and follicular lymphoma. In 4 cases, monoclonality was detected definitively only by evaluating the immunoglobulin light chain restriction in B cells with aberrant CD22 expression because numerous polyclonal B cells were present. Aberrant CD22 expression is a useful marker for detection of monoclonal B cells admixed with numerous benign polyclonal B cells.

MeSH terms

  • Antigens, CD / biosynthesis*
  • Antigens, Differentiation, B-Lymphocyte / biosynthesis*
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / metabolism
  • Biomarkers, Tumor / analysis*
  • Cell Adhesion Molecules / biosynthesis*
  • Cell Differentiation
  • Flow Cytometry
  • Humans
  • Immunoglobulin Light Chains / immunology
  • Immunophenotyping
  • Lectins / biosynthesis*
  • Leukemia, Lymphocytic, Chronic, B-Cell / diagnosis*
  • Leukemia, Lymphocytic, Chronic, B-Cell / immunology
  • Lymphoma, B-Cell / diagnosis*
  • Lymphoma, B-Cell / immunology
  • Retrospective Studies
  • Sialic Acid Binding Ig-like Lectin 2

Substances

  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • Biomarkers, Tumor
  • CD22 protein, human
  • Cell Adhesion Molecules
  • Immunoglobulin Light Chains
  • Lectins
  • Sialic Acid Binding Ig-like Lectin 2