The novel gene fad104, containing a fibronectin type III domain, has a significant role in adipogenesis

FEBS Lett. 2004 Nov 5;577(1-2):49-54. doi: 10.1016/j.febslet.2004.09.062.

Abstract

A novel gene named fad104 (factor for adipocyte differentiation-104), whose expression level quickly increased in the early stage of adipogenesis, was isolated and characterized. The deduced amino acid sequence of fad104 revealed the possible presence of a fibronectin type III domain and transmembrane domain. The expression of fad104 was detected in adipocyte differentiable 3T3-L1 cells but not observed in the non-adipogenic cell line NIH-3T3. Moreover, the ability of 3T3-L1 cells to differentiate declined with the knockdown of fad104 by RNA interference, strongly indicating that fad104 functions as a positive regulator of adipogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / cytology*
  • Animals
  • Base Sequence
  • Cell Differentiation / genetics*
  • DNA, Complementary
  • Fibronectins / genetics*
  • Humans
  • Mice
  • NIH 3T3 Cells
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • DNA, Complementary
  • Fibronectins