Abstract
The tumor suppressor gene DICE1 is located within a previously reported critical region of loss of heterozygosity on chromosome 13q14.3. Expression of the remaining DICE1 allele is down-regulated in non-small cell lung carcinomas. Ectopic expression of DICE1 cDNA by DICE1-green fluorescent protein fusion constructs resulted in inhibition of colony formation of human non-small cell lung carcinoma cell line SK-MES-1 and NCI-H520 and prostate carcinoma cell line DU145. In IGF-IR transformed Balb/c 3T3, DICE1 substantially sup-pressed growth in soft agar. These results demonstrate that DICE1 has a growth-suppressing activity and interferes with anchorage-independent growth of IGF-IR transformed tumor cells dependent upon IGF-I signaling.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Agar / chemistry
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Amino Acid Motifs
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Animals
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Blotting, Northern
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Cell Line, Tumor
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Cell Proliferation
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DNA, Complementary / metabolism
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Exons
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Genes, Tumor Suppressor
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Green Fluorescent Proteins / metabolism
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Humans
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Insulin-Like Growth Factor I / metabolism
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Mice
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Mice, Inbred BALB C
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Neoplasms / metabolism*
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Plasmids / metabolism
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RNA / metabolism
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RNA Helicases / biosynthesis*
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RNA, Messenger / metabolism
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RNA-Binding Proteins
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Recombinant Fusion Proteins / metabolism
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Ribosomal Proteins
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Signal Transduction
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Tumor Suppressor Proteins / biosynthesis*
Substances
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DNA, Complementary
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INTS6 protein, human
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RNA, Messenger
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RNA-Binding Proteins
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Recombinant Fusion Proteins
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Ribosomal Proteins
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Tumor Suppressor Proteins
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Green Fluorescent Proteins
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RNA
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Insulin-Like Growth Factor I
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Agar
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RNA Helicases