Dorz1, a novel gene expressed in differentiating cerebellar granule neurons, is down-regulated in Zic1-deficient mouse

Jun Hoshino; Jun Aruga; Akira Ishiguro; Katsuhiko Mikoshiba

doi:10.1016/j.molbrainres.2003.10.004

Dorz1, a novel gene expressed in differentiating cerebellar granule neurons, is down-regulated in Zic1-deficient mouse

Brain Res Mol Brain Res. 2003 Dec 12;120(1):57-64. doi: 10.1016/j.molbrainres.2003.10.004.

Authors

Jun Hoshino¹, Jun Aruga, Akira Ishiguro, Katsuhiko Mikoshiba

Affiliation

¹ Laboratory for Developmental Neurobiology, RIKEN Brain Science Institute (BSI), 2-1 Hirosawa, Wako, Saitama 351-0918, Japan.

PMID: 14667578
DOI: 10.1016/j.molbrainres.2003.10.004

Abstract

The Zic1 gene encodes a zinc finger protein that controls vertebrate neural development. A previous study showed that Zic1 is expressed in developing and mature cerebellar granule neurons, and that Zic1-deficient cerebellum is hypoplastic and lacks a lobule of the anterior lobe. In the present study, we searched for genes de-regulated in the cerebellum to understand the molecular basis of cerebellar development. A novel gene, Dorz1, was identified and characterized as one of the most significantly down-regulated genes in Zic1-deficient cerebellum by the DNA microarray analysis. The expression of Dorz1 in the developing cerebellum peaked at embryonic day 17.5, and at that stage Dorz1 transcripts were detected in cerebellar granule neuron precursors where Zic1 expression is enhanced. In addition, Dorz1 expression was up-regulated in cultured cells overexpressing Zic1. These results suggest that Dorz1 expression is positively regulated by Zic1 during cerebellar development.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Aging
Amino Acid Sequence
Animals
Animals, Newborn
Blotting, Northern
Blotting, Western
Brain / anatomy & histology
Brain / metabolism
Cells, Cultured
Cerebellum* / cytology*
Cerebellum* / metabolism
Cloning, Molecular
DNA-Binding Proteins / metabolism*
Down-Regulation / genetics*
Embryo, Mammalian
Gene Expression Regulation, Developmental*
Hydrolases
Immunohistochemistry
In Situ Hybridization
Indoles / metabolism
Membrane Proteins
Mice
Mice, Neurologic Mutants
NIH 3T3 Cells
Neurons / classification
Neurons / metabolism*
Oligonucleotide Array Sequence Analysis / methods
Proteins / metabolism
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction / methods
Sequence Alignment
Transcription Factors / genetics
Transcription Factors / immunology
Transcription Factors / metabolism*
Transfection

Substances

ABHD14A protein, human
DNA-Binding Proteins
Dorz1 protein, mouse
Indoles
Membrane Proteins
Proteins
RNA, Messenger
Transcription Factors
ZIC1 protein, human
Zic1 protein, mouse
DAPI
Hydrolases