Abstract
Expression of VEGF and VEGFR support a role for angiogenic pathways in the pathogenesis of some hematological malignances. Our goal was to determine if expression of these angiogenic molecules also extend to childhood precursor B cell acute lymphoblastic leukemia (pre-B ALL). We now show that transcripts of VEGF, and its receptors VEGFR-1 and VEGFR-2 are concomitantly expressed in both ALL cell lines and primary pre-B ALL. Western blot and ELISA consistently detected VEGF protein in the supernatants of the cell lines. Similarly, VEGFR-1 and VEGFR-2 proteins are also detectable by FACS analysis. Interestingly, the expression of the receptors in immature B cells is limited to the intra-cytoplasmic compartment and may suggest either internalization of the receptors or a block in trafficking of the receptor to the surface.
MeSH terms
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Autocrine Communication
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B-Lymphocytes / metabolism
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B-Lymphocytes / pathology*
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Blood Cells / pathology
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Bone Marrow / pathology
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Burkitt Lymphoma / etiology
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Burkitt Lymphoma / metabolism*
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Burkitt Lymphoma / pathology
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Cell Line, Tumor
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Cytoplasm / chemistry
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Humans
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Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / etiology
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Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / metabolism
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Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / pathology
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RNA, Messenger / analysis
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Vascular Endothelial Growth Factor A / biosynthesis*
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Vascular Endothelial Growth Factor Receptor-1 / analysis
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Vascular Endothelial Growth Factor Receptor-1 / biosynthesis*
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Vascular Endothelial Growth Factor Receptor-2 / analysis
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Vascular Endothelial Growth Factor Receptor-2 / biosynthesis*
Substances
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RNA, Messenger
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factor Receptor-1
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Vascular Endothelial Growth Factor Receptor-2