Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase

Biochem Biophys Res Commun. 1992 Dec 15;189(2):749-58. doi: 10.1016/0006-291x(92)92265-y.

Abstract

We have cloned and sequenced a full-length cDNA (1083 bp) encoding the human liver cystathionine-gamma-lyase enzyme (cystathionase). The human cystathionase sequence presented a substantial deletion of 132 bases (44 amino acids) compared to that reported for rat cystathionase, and of 135 bases (45 amino acids) compared to that reported for yeast cystathionase. After re-alignment for the missing nucleotides, the human cDNA sequence shows significant amino acid homology to that for the rat enzyme (85%) and the yeast enzyme (50%). A search for an undeleted cDNA, by the polymerase chain reaction, yielded a second clone which contained the missing 132 bases. Flanking nucleotides in the latter clone were identical to those in the cDNA clone containing the deletion. The two forms of human cystathionase deduced from the two cDNA clones may be derived from two different genes or may be splice variants.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cloning, Molecular
  • Cystathionine gamma-Lyase / genetics*
  • DNA / genetics*
  • DNA / isolation & purification
  • Gene Library
  • Humans
  • Liver / enzymology*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • RNA, Messenger / metabolism
  • Rats
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics
  • Sequence Deletion
  • Sequence Homology, Amino Acid

Substances

  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • DNA
  • Cystathionine gamma-Lyase

Associated data

  • GENBANK/M86474
  • GENBANK/M86475
  • GENBANK/M86476
  • GENBANK/M86477
  • GENBANK/M86478
  • GENBANK/M86479
  • GENBANK/M86480
  • GENBANK/M86481
  • GENBANK/S52028
  • GENBANK/S52784