Thymic medullary epithelial cell differentiation, thymocyte emigration, and the control of autoimmunity require lympho-epithelial cross talk via LTbetaR

J Exp Med. 2003 Sep 1;198(5):757-69. doi: 10.1084/jem.20030794.

Abstract

Thymocytes depend on the interaction with thymic epithelial cells for the generation of a diverse, nonautoreactive T cell repertoire. In turn, thymic epithelial cells acquire their three-dimensional cellular organization via instructive signals from developing thymocytes. The nature of these signals has been elusive so far. We show that thymocytes and medullary epithelial cells (MECs) communicate via the lymphotoxin beta receptor (LTbetaR) signaling axis. Normal differentiation of thymic MECs requires LTbetaR ligand on thymocytes and LTbetaR together with nuclear factor-kappaB-inducing kinase (Nik) in thymic epithelial cells. Impaired lympho-epithelial cross talk in the absence of the LTbetaR causes aberrant differentiation and reduced numbers of thymic MECs, leads to the retention of mature T lymphocytes, and is associated with autoimmune phenomena, suggesting an unexpected role for LTbetaR signaling in central tolerance induction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoimmunity
  • Cell Differentiation
  • Epithelial Cells / cytology*
  • Epithelial Cells / immunology
  • Flow Cytometry
  • Ligands
  • Lymphotoxin beta Receptor
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Receptor Cross-Talk / immunology*
  • Receptors, Tumor Necrosis Factor / deficiency
  • Receptors, Tumor Necrosis Factor / genetics
  • Receptors, Tumor Necrosis Factor / immunology*
  • Recombinant Fusion Proteins / immunology
  • Reference Values
  • T-Lymphocytes / immunology*
  • Thymus Gland / cytology*
  • Thymus Gland / immunology*

Substances

  • Ligands
  • Ltbr protein, mouse
  • Lymphotoxin beta Receptor
  • Receptors, Tumor Necrosis Factor
  • Recombinant Fusion Proteins