Time- and pH-dependent release of PDGF and TGF-beta from platelets in vitro

Platelets. 2003 Jun;14(4):233-7. doi: 10.1080/0953710031000118876.

Abstract

We studied the spontaneous and thrombin-induced activation of platelets and their release of platelet derived growth factor (PDGF) and transforming growth factor-beta (TGF-beta) at different pH values. Platelet activation was assayed with antibodies against P-selectin and performed in serum-free media. The release of PDGF and TGF-beta was determined by ELISA after 15 min and 12h. There was no activation at pH 5.0, while a time-dependent release of growth factors occurred at neutral and alkaline pH. The results suggest that release o f growth factors is not only dependent on platelet activation but also on incubation time and pH. Although the used serum-free experimental situation is different from normal conditions for platelets in vivo, the findings of a late release of growth factors may, nevertheless, be relevant to wound healing.

MeSH terms

  • Blood Platelets / metabolism*
  • Cells, Cultured
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Hydrogen-Ion Concentration
  • Platelet Activation
  • Platelet-Derived Growth Factor / analysis
  • Platelet-Derived Growth Factor / metabolism*
  • Thrombin
  • Time Factors
  • Transforming Growth Factor beta / analysis
  • Transforming Growth Factor beta / metabolism*
  • Wound Healing

Substances

  • Platelet-Derived Growth Factor
  • Transforming Growth Factor beta
  • Thrombin