Abstract
Type B T cells recognize peptide provided exogenously but are ignorant of the same epitope derived from intracellular processing. In this study, we demonstrate the existence of type B T cells to an abundant autologous peptide derived from processing of the I-A(k) beta-chain. T cell hybridomas raised against this peptide fail to recognize syngeneic APC despite abundant presentation of the naturally processed epitope but react in a dose-dependent manner to exogenous peptide. Moreover, these hybridomas respond to Abeta(k) peptide extracted from the surface of I-A(k)-expressing APC. This peptide was isolated from B cell lines where it was found in high abundance; it was also present in lines lacking HLA-DM, but in considerably lower amounts. Therefore, type B T cells exist in the naive repertoire to abundant autologous peptides. We discuss the implications of these findings to the potential biological role of type B T cells in immune responses and autoimmune pathology.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigen Presentation / immunology*
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Antigen-Presenting Cells / immunology*
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Antigen-Presenting Cells / metabolism
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Autoantigens / administration & dosage
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Autoantigens / biosynthesis
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Autoantigens / immunology*
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Autoantigens / metabolism
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B-Lymphocyte Subsets / classification
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B-Lymphocyte Subsets / immunology
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B-Lymphocyte Subsets / metabolism
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Cell Line
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Cell Membrane / immunology
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Cell Membrane / metabolism
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Epitopes, T-Lymphocyte / immunology
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Epitopes, T-Lymphocyte / metabolism
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Histocompatibility Antigens Class II / administration & dosage
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Histocompatibility Antigens Class II / biosynthesis
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Histocompatibility Antigens Class II / immunology*
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Histocompatibility Antigens Class II / metabolism
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Hybridomas
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Multigene Family / immunology
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Peptide Fragments / administration & dosage
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Peptide Fragments / biosynthesis
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Peptide Fragments / immunology*
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Peptide Fragments / metabolism
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Protein Processing, Post-Translational / immunology
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T-Lymphocyte Subsets / immunology*
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T-Lymphocyte Subsets / metabolism
Substances
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Autoantigens
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Epitopes, T-Lymphocyte
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Histocompatibility Antigens Class II
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I-A(b) antigen, mouse
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I-Ak antigen
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Peptide Fragments