Abstract
Bone morphogenetic protein-15 (BMP-15) has been known to be an oocyte-specific factor and regulate granulosa cell functions. Here, we report that BMP-15 is also expressed in the well established gonadotrope cell line, LbetaT2 and mouse pituitary tissue. Interestingly, BMP-15 stimulates FSHbeta subunit transcription in LbetaT2 cells without affecting LHbeta and GnRH receptor transcription. These findings, therefore, suggest a possible autocrine control mechanism selective for FSHbeta synthesis by BMP-15. Moreover, BMP-15 exerts a potent and selective stimulation of FSH, but not LH, secretion by primary pituitary cells. Taken together, we hypothesize that BMP-15 could play a physiological role in the monotropic rise of FSH secretion by the pituitary during the estrous and menstrual cycle. These findings, together with the previous evidence of a negative regulation of FSH receptor expression by BMP-15 in the ovary, may constitute a major role in female fertility.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Activins / pharmacology
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Animals
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Bone Morphogenetic Protein 15
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Bone Morphogenetic Protein 6
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Bone Morphogenetic Protein 7
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Bone Morphogenetic Proteins / pharmacology
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Cell Line
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Cells, Cultured
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Female
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Follicle Stimulating Hormone / biosynthesis*
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Follicle Stimulating Hormone / metabolism*
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Follicle Stimulating Hormone, beta Subunit / genetics
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Gene Expression
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Gene Expression Regulation / drug effects
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Growth Differentiation Factor 9
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Inhibin-beta Subunits / pharmacology
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Intercellular Signaling Peptides and Proteins / genetics
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Intercellular Signaling Peptides and Proteins / pharmacology
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Intercellular Signaling Peptides and Proteins / physiology*
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Luteinizing Hormone / biosynthesis
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Luteinizing Hormone / metabolism
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Luteinizing Hormone, beta Subunit / genetics
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Male
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Mice
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Ovary / chemistry
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Pituitary Gland / chemistry
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Pituitary Gland / physiology*
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Pituitary Gland, Anterior / metabolism
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RNA, Messenger / analysis
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Rats
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Rats, Sprague-Dawley
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Receptors, FSH / genetics
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Receptors, LHRH / genetics
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Transcription, Genetic / drug effects
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Transforming Growth Factor beta*
Substances
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Bmp15 protein, mouse
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Bmp15 protein, rat
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Bmp6 protein, mouse
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Bmp6 protein, rat
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Bmp7 protein, rat
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Bone Morphogenetic Protein 15
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Bone Morphogenetic Protein 6
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Bone Morphogenetic Protein 7
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Bone Morphogenetic Proteins
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Follicle Stimulating Hormone, beta Subunit
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Gdf9 protein, mouse
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Gdf9 protein, rat
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Growth Differentiation Factor 9
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Intercellular Signaling Peptides and Proteins
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Luteinizing Hormone, beta Subunit
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RNA, Messenger
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Receptors, FSH
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Receptors, LHRH
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Transforming Growth Factor beta
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activin A
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Activins
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Luteinizing Hormone
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Follicle Stimulating Hormone
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Inhibin-beta Subunits