cDNA cloning, genomic structure and chromosomal mapping of the mouse glucuronyltransferase-S involved in the biosynthesis of the HNK-1 carbohydrate epitope

Gene. 2002 Aug 21;296(1-2):29-36. doi: 10.1016/s0378-1119(02)00840-5.

Abstract

The HNK-1 carbohydrate epitope is expressed on a series of cell adhesion molecules and some glycolipids in the nervous system. Two glucuronyltransferases (GlcAT-P and GlcAT-S) are involved in the biosynthesis of the HNK-1 carbohydrate epitope. In this study, we isolated cDNA and genomic clones encoding the mouse glucuronyltransferase-S involved in the biosynthesis of the HNK-1 carbohydrate epitope and determined the structural organization of the gene. The deduced amino acid sequence of mouse GlcAT-S consists of 324 amino acids and has a type II membrane topology. The predicted amino acid sequence of mouse GlcAT-S is 98.1% identical to that of rat GlcAT-S. Northern blot analysis revealed that the mouse GlcAT-S transcript is specifically expressed in the nervous system. Moreover, the mouse GlcAT-S gene is composed of four exons spanning over more than 25 kilobase pairs. Southern blot analysis and chromosomal mapping indicated that the mouse GlcAT-S gene is a single copy gene and it was mapped to the A4-B region of mouse chromosome 1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Flanking Region / genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • CD57 Antigens / metabolism*
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics*
  • Epitopes / biosynthesis*
  • Exons
  • Gene Expression Regulation, Enzymologic
  • Genes / genetics
  • Glucuronosyltransferase / genetics*
  • Glucuronosyltransferase / metabolism
  • Introns
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Regulatory Sequences, Nucleic Acid / genetics
  • Sequence Analysis, DNA
  • Transcription Initiation Site

Substances

  • CD57 Antigens
  • DNA, Complementary
  • Epitopes
  • Isoenzymes
  • RNA, Messenger
  • Glucuronosyltransferase

Associated data

  • GENBANK/AB055902
  • GENBANK/AB055903