Abstract
The extracellular moiety of the hemoglobin/haptoglobin scavenger receptor CD163 (RM3/1 antigen) can be shed from monocytes and is a normal plasma component. We found that in a dose-dependent manner soluble CD163 induces a decrease in CD69 expression, a reduced [(3)H]thymidine uptake and a down-regulated matrix metalloproteinase-9 RNA expression in phorbol myristate acetate-stimulated T-cells. Co-culturing T-cells on transgenic fibroblasts, expressing membrane-bound CD163, yielded no differences compared to culture on non-transfected cells. We conclude that CD163 has at least two distinct functions: the clearance of hemoglobin in its cell-bound form and participation in anti-inflammation as a soluble factor, exhibiting cytokine-like functions.
MeSH terms
-
Antigens, CD / genetics
-
Antigens, Differentiation, Myelomonocytic / genetics
-
Antigens, Differentiation, Myelomonocytic / isolation & purification
-
Antigens, Differentiation, Myelomonocytic / physiology*
-
Antigens, Differentiation, T-Lymphocyte / genetics
-
Apoptosis
-
Chromatography, Affinity
-
Gene Expression Regulation
-
Humans
-
Lectins, C-Type
-
Lymphocyte Activation / drug effects
-
Lymphocyte Activation / physiology*
-
Matrix Metalloproteinase 3 / genetics
-
Matrix Metalloproteinase 9 / genetics
-
Receptors, Cell Surface / genetics
-
Receptors, Cell Surface / isolation & purification
-
Receptors, Cell Surface / physiology*
-
Recombinant Proteins / isolation & purification
-
Recombinant Proteins / metabolism
-
Reverse Transcriptase Polymerase Chain Reaction
-
T-Lymphocytes / drug effects
-
T-Lymphocytes / immunology*
-
Tetradecanoylphorbol Acetate
Substances
-
Antigens, CD
-
Antigens, Differentiation, Myelomonocytic
-
Antigens, Differentiation, T-Lymphocyte
-
CD163 antigen
-
CD69 antigen
-
Lectins, C-Type
-
Receptors, Cell Surface
-
Recombinant Proteins
-
Matrix Metalloproteinase 3
-
Matrix Metalloproteinase 9
-
Tetradecanoylphorbol Acetate