Only the soluble form of the scavenger receptor CD163 acts inhibitory on phorbol ester-activated T-lymphocytes, whereas membrane-bound protein has no effect

FEBS Lett. 2002 Aug 28;526(1-3):93-6. doi: 10.1016/s0014-5793(02)03142-3.

Abstract

The extracellular moiety of the hemoglobin/haptoglobin scavenger receptor CD163 (RM3/1 antigen) can be shed from monocytes and is a normal plasma component. We found that in a dose-dependent manner soluble CD163 induces a decrease in CD69 expression, a reduced [(3)H]thymidine uptake and a down-regulated matrix metalloproteinase-9 RNA expression in phorbol myristate acetate-stimulated T-cells. Co-culturing T-cells on transgenic fibroblasts, expressing membrane-bound CD163, yielded no differences compared to culture on non-transfected cells. We conclude that CD163 has at least two distinct functions: the clearance of hemoglobin in its cell-bound form and participation in anti-inflammation as a soluble factor, exhibiting cytokine-like functions.

MeSH terms

  • Antigens, CD / genetics
  • Antigens, Differentiation, Myelomonocytic / genetics
  • Antigens, Differentiation, Myelomonocytic / isolation & purification
  • Antigens, Differentiation, Myelomonocytic / physiology*
  • Antigens, Differentiation, T-Lymphocyte / genetics
  • Apoptosis
  • Chromatography, Affinity
  • Gene Expression Regulation
  • Humans
  • Lectins, C-Type
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / physiology*
  • Matrix Metalloproteinase 3 / genetics
  • Matrix Metalloproteinase 9 / genetics
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / isolation & purification
  • Receptors, Cell Surface / physiology*
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology*
  • Tetradecanoylphorbol Acetate

Substances

  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • Antigens, Differentiation, T-Lymphocyte
  • CD163 antigen
  • CD69 antigen
  • Lectins, C-Type
  • Receptors, Cell Surface
  • Recombinant Proteins
  • Matrix Metalloproteinase 3
  • Matrix Metalloproteinase 9
  • Tetradecanoylphorbol Acetate