A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells

Science. 2002 May 10;296(5570):1132-6. doi: 10.1126/science.1069861.

Abstract

E2F-6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. To better elucidate the molecular mechanism of repression by E2F-6, we have purified the factor from cultured cells. E2F-6 is found in a multimeric protein complex that contains Mga and Max, and thus the complex can bind not only to the E2F-binding site but also to Myc- and Brachyury-binding sites. Moreover, the complex contains chromatin modifiers such as a novel histone methyltransferase that modifies lysine 9 of histone H3, HP1gamma, and Polycomb group (PcG) proteins. The E2F-6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells. These data suggest that these chromatin modifiers contribute to silencing of E2F- and Myc-responsive genes in quiescent cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Cell Cycle Proteins*
  • Chromatin / metabolism*
  • Chromobox Protein Homolog 5
  • Chromosomal Proteins, Non-Histone / metabolism
  • DNA-Binding Proteins*
  • Dimerization
  • E2F Transcription Factors
  • E2F6 Transcription Factor
  • G1 Phase
  • Gene Silencing*
  • HeLa Cells
  • Histone Methyltransferases
  • Histone-Lysine N-Methyltransferase*
  • Histones / metabolism
  • Humans
  • Mass Spectrometry
  • Methylation
  • Methyltransferases / chemistry
  • Methyltransferases / metabolism
  • Molecular Sequence Data
  • Phosphoproteins / metabolism
  • Promoter Regions, Genetic*
  • Protein Footprinting
  • Protein Methyltransferases
  • Proteins*
  • Proto-Oncogene Proteins c-myc / metabolism
  • Recombinant Proteins / metabolism
  • Resting Phase, Cell Cycle*
  • Retinoblastoma-Like Protein p130
  • Transcription Factors / chemistry
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism*
  • Transcription, Genetic*
  • Two-Hybrid System Techniques

Substances

  • Cell Cycle Proteins
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins
  • E2F Transcription Factors
  • E2F6 Transcription Factor
  • E2F6 protein, human
  • Histones
  • Phosphoproteins
  • Proteins
  • Proto-Oncogene Proteins c-myc
  • RBL2 protein, human
  • Recombinant Proteins
  • Retinoblastoma-Like Protein p130
  • Transcription Factors
  • Chromobox Protein Homolog 5
  • EHMT1 protein, human
  • Histone Methyltransferases
  • Methyltransferases
  • Protein Methyltransferases
  • Histone-Lysine N-Methyltransferase