Expression and localization of the multidrug resistance-associated protein 3 in rat small and large intestine

Am J Physiol Gastrointest Liver Physiol. 2002 Apr;282(4):G720-6. doi: 10.1152/ajpgi.00318.2001.

Abstract

Multidrug resistance-associated protein 3 (MRP3; symbol ABCC3), has been shown to mediate ATP-dependent transport of organic anions including 17beta-glucuronosyl estradiol, glucuronosyl bilirubin, monovalent, and sulfated bile salts. MRP3 mRNA expression was reported in rat intestine suggesting a role of MRP3 in the intestinal transport. We examined the expression and localization of MRP3 in rat small and large intestine by RT-PCR, immunofluorescence, and immunoblot analysis. MRP3 was identified in all intestinal segments by RT-PCR. MRP3 expression was low in duodenum and jejunum but markedly increased in ileum and colon. With the use of a rat MRP3 specific antibody, MRP3 was localized to the basolateral domains of enterocytes. Immunofluorescence analysis and immunoblot analysis confirmed a strong expression of rat MRP3 in ileum and colon. In contrast, MRP2 was predominantly expressed in the proximal segments of rat small intestine. Our findings demonstrate a high expression of rat MRP3 in ileum and colon and provide evidence for an involvement of MRP3 in the ATP-dependent transport of organic anions, including bile salts from the enterocyte into blood.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Colon / chemistry
  • Duodenum / chemistry
  • Fluorescent Antibody Technique
  • Gene Expression*
  • Ileum / chemistry
  • Immunoblotting
  • Intestinal Mucosa / chemistry
  • Intestinal Mucosa / metabolism
  • Intestines / chemistry*
  • Jejunum / chemistry
  • Male
  • Microscopy, Fluorescence
  • Mitochondrial Proteins*
  • Multidrug Resistance-Associated Proteins / analysis*
  • Multidrug Resistance-Associated Proteins / genetics*
  • Rats
  • Rats, Wistar
  • Reverse Transcriptase Polymerase Chain Reaction
  • Ribosomal Proteins / genetics
  • Saccharomyces cerevisiae Proteins*
  • Tissue Distribution

Substances

  • MRP2 protein, S cerevisiae
  • Mitochondrial Proteins
  • Multidrug Resistance-Associated Proteins
  • Ribosomal Proteins
  • Saccharomyces cerevisiae Proteins
  • multidrug resistance-associated protein 3