Abstract
CCR9 mediates chemotaxis in response to CCL25/thymus-expressed chemokine and is selectively expressed on T cells in the thymus and small intestine. To investigate the role of CCR9 in T cell development, the CCR9 gene was disrupted by homologous recombination. B cell development, thymic alphabeta-T cell development, and thymocyte selection appeared unimpaired in adult CCR9-deficient (CCR9(-/-)) mice. However, competitive transplantation experiments revealed that bone marrow from CCR9(-/-) mice was less efficient at repopulating the thymus of lethally irradiated Rag-1(-/-) mice than bone marrow from littermate CCR9(+/+) mice. CCR9(-/-) mice had increased numbers of peripheral gammadelta-T cells but reduced numbers of gammadeltaTCR(+) and CD8alphabeta(+)alphabetaTCR(+) intraepithelial lymphocytes in the small intestine. Thus, CCR9 plays an important, although not indispensable, role in regulating the development and/or migration of both alphabeta(-) and gammadelta(-) T lymphocytes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aging / genetics
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Aging / immunology
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Animals
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B-Lymphocyte Subsets / cytology
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Bone Marrow Cells / immunology
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Bone Marrow Cells / metabolism
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Bone Marrow Cells / pathology
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Cells, Cultured
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Chemotaxis, Leukocyte / genetics
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Chemotaxis, Leukocyte / immunology*
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Crosses, Genetic
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Hematopoiesis / immunology
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Intestinal Mucosa / immunology
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Intestinal Mucosa / metabolism
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Lymph Nodes / cytology
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Lymph Nodes / immunology
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Lymph Nodes / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Organ Specificity / genetics
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Organ Specificity / immunology
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Receptors, Antigen, T-Cell, alpha-beta / biosynthesis
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Receptors, Antigen, T-Cell, gamma-delta / biosynthesis
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Receptors, CCR
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Receptors, Chemokine / biosynthesis
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Receptors, Chemokine / deficiency
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Receptors, Chemokine / genetics
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Receptors, Chemokine / physiology*
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Spleen / cytology
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Spleen / immunology
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Spleen / metabolism
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T-Lymphocyte Subsets / cytology*
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T-Lymphocyte Subsets / immunology*
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T-Lymphocyte Subsets / metabolism
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T-Lymphocyte Subsets / pathology
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Thymus Gland / immunology
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Thymus Gland / pathology
Substances
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CC chemokine receptor 9
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Receptors, Antigen, T-Cell, alpha-beta
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Receptors, Antigen, T-Cell, gamma-delta
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Receptors, CCR
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Receptors, Chemokine