Objective: To investigate the gene frequencies of 5 major human platelet alloantiqens (HPA 1-5) in Chinese population and to assess if polymorphism of HPA was associated with Chinese acute myocardial infarction (AMI) and acute ischemic stroke (AIS).
Methods: HPA 1-5 genotyping was performed by PCR using allele specific primers and restriction enzyme digestion based on PCR products in 95 AMI cases, 188 AIS cases and 270 normal controls. Gene frequency distribution was tested by Hardy-Weinberg equilibrium and comparison of HPA gene frequencies between the patient and control groups by chi 2 test.
Results: The gene frequencies of HPA 1-5 were the followings: HPA1a: 91%; 1b: 9%; HPA 2a: 94%; 2b: 6%; HPA 3a: 83%; 3b: 17%; HPA 4a: 98%; 4b: 2%; HPA5a: 97%; 5b: 3%. We found there were no significant differences in HPA 1-5 gene frequencies between AMI patients and normal controls. In AIS patients group HPA-2a allele frequency was significant higher than in controls, but this allele gene frequency in two groups (0.94 and 0.99) was very close and too many subjects in these two groups were overlapped. Otherwise no differences was found in other 4 HPA systems between cases and controls.
Conclusion: Polymorphism of HPA were not inherited risky factors and not associated with chinese arterial thrombotic diseases such as AMI and AIS.