Histamine increases the expression of LOX-1 via H2 receptor in human monocytic THP-1 cells

FEBS Lett. 2001 Nov 23;508(3):345-9. doi: 10.1016/s0014-5793(01)03073-3.

Abstract

Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a member of the scavenger receptor family, and is known to be expressed in monocytes/macrophages. We investigated the effect of histamine on the expression of LOX-1 in cells of the human monocytic leukemia cell line THP-1. Histamine as well as forskolin and dibutyryl cyclic AMP (Bt2-cAMP) stimulated the THP-1 monocytes to express the LOX-1 gene at the transcription level. This histamine effect on LOX-1 gene expression, via the histamine H2 receptor-mediated cAMP signal transduction pathway, was reduced after differentiation of the cells into macrophages, even though forskolin and Bt2-cAMP still enhanced the gene expression. The alteration of the responsiveness of LOX-1 expression to histamine was related to suppressed expression of the H2 receptor in THP-1 macrophages. The switch of the predominant class of histamine receptors between H1 and H2 would modulate the effects of histamine on LOX-1 gene expression in monocytes and macrophages, and therefore, would play a certain role in the inflammatory aspects of atherogenesis.

MeSH terms

  • Bucladesine / pharmacology
  • CREB-Binding Protein
  • Cell Differentiation
  • Colforsin / pharmacology
  • Cyclic AMP / metabolism
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • Cytokines / metabolism
  • Dinoprostone / pharmacology
  • Enzyme Inhibitors / pharmacology
  • Gene Expression Regulation / drug effects*
  • Histamine / pharmacology*
  • Humans
  • Isoquinolines / pharmacology
  • Macrophages / cytology
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Monocytes / cytology
  • Monocytes / drug effects
  • Monocytes / metabolism*
  • Nuclear Proteins / metabolism
  • Promoter Regions, Genetic / drug effects
  • Prostaglandin D2 / pharmacology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Histamine H1 / genetics
  • Receptors, Histamine H1 / metabolism
  • Receptors, Histamine H2 / genetics
  • Receptors, Histamine H2 / metabolism*
  • Receptors, LDL / biosynthesis
  • Receptors, LDL / genetics*
  • Receptors, Oxidized LDL
  • Scavenger Receptors, Class E
  • Signal Transduction
  • Sulfonamides*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Trans-Activators / metabolism
  • Tumor Cells, Cultured
  • Up-Regulation

Substances

  • Cytokines
  • Enzyme Inhibitors
  • Isoquinolines
  • Nuclear Proteins
  • OLR1 protein, human
  • RNA, Messenger
  • Receptors, Histamine H1
  • Receptors, Histamine H2
  • Receptors, LDL
  • Receptors, Oxidized LDL
  • Scavenger Receptors, Class E
  • Sulfonamides
  • Trans-Activators
  • Colforsin
  • Bucladesine
  • Histamine
  • Cyclic AMP
  • CREB-Binding Protein
  • CREBBP protein, human
  • Cyclic AMP-Dependent Protein Kinases
  • Dinoprostone
  • N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide
  • Tetradecanoylphorbol Acetate
  • Prostaglandin D2