CD147 is a signaling receptor for cyclophilin B

Biochem Biophys Res Commun. 2001 Nov 9;288(4):786-8. doi: 10.1006/bbrc.2001.5847.

Abstract

Cyclophilins A and B (CyPA and CyPB) are cyclosporin A binding proteins that can be secreted in response to inflammatory stimuli. We recently identified CD147 as a cell-surface receptor for CyPA and demonstrated that CD147 is an essential component in the CyPA-initiated signaling cascade that culminates in ERK activation and chemotaxis. Here we demonstrate that CD147 also serves as a receptor for CyPB. CyPB induced Ca(2+) flux and chemotaxis of CD147-transfected, but not control, CHO cells, and the chemotactic response of primary human neutrophils to CyPB was blocked by antibodies to CD147. These results suggest that CD147 serves as a receptor for extracellular cyclophilins.

MeSH terms

  • Animals
  • Antibodies / immunology
  • Antibodies / pharmacology
  • Antigens, CD*
  • Antigens, Neoplasm*
  • Antigens, Surface*
  • Avian Proteins*
  • Basigin
  • Blood Proteins*
  • CHO Cells
  • Calcium Signaling / drug effects*
  • Cells, Cultured
  • Chemotaxis / drug effects
  • Cricetinae
  • Cyclophilin A / pharmacology
  • Cyclophilins / pharmacology*
  • Enzyme Activation / drug effects
  • Humans
  • Membrane Glycoproteins / antagonists & inhibitors
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / immunology
  • Membrane Glycoproteins / metabolism*
  • Mitogen-Activated Protein Kinases / metabolism
  • Neutrophil Infiltration / drug effects
  • Neutrophils / drug effects
  • Neutrophils / enzymology
  • Neutrophils / metabolism
  • Peptidylprolyl Isomerase
  • Phosphorylation / drug effects

Substances

  • Antibodies
  • Antigens, CD
  • Antigens, Neoplasm
  • Antigens, Surface
  • Avian Proteins
  • BSG protein, human
  • Blood Proteins
  • Bsg protein, Gallus gallus
  • Bsg protein, rat
  • Membrane Glycoproteins
  • Basigin
  • cyclophilin B
  • Mitogen-Activated Protein Kinases
  • Cyclophilin A
  • Cyclophilins
  • Peptidylprolyl Isomerase