Abstract
Cyclophilins A and B (CyPA and CyPB) are cyclosporin A binding proteins that can be secreted in response to inflammatory stimuli. We recently identified CD147 as a cell-surface receptor for CyPA and demonstrated that CD147 is an essential component in the CyPA-initiated signaling cascade that culminates in ERK activation and chemotaxis. Here we demonstrate that CD147 also serves as a receptor for CyPB. CyPB induced Ca(2+) flux and chemotaxis of CD147-transfected, but not control, CHO cells, and the chemotactic response of primary human neutrophils to CyPB was blocked by antibodies to CD147. These results suggest that CD147 serves as a receptor for extracellular cyclophilins.
Copyright 2001 Academic Press.
MeSH terms
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Animals
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Antibodies / immunology
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Antibodies / pharmacology
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Antigens, CD*
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Antigens, Neoplasm*
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Antigens, Surface*
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Avian Proteins*
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Basigin
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Blood Proteins*
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CHO Cells
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Calcium Signaling / drug effects*
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Cells, Cultured
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Chemotaxis / drug effects
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Cricetinae
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Cyclophilin A / pharmacology
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Cyclophilins / pharmacology*
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Enzyme Activation / drug effects
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Humans
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Membrane Glycoproteins / antagonists & inhibitors
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / immunology
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Membrane Glycoproteins / metabolism*
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Mitogen-Activated Protein Kinases / metabolism
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Neutrophil Infiltration / drug effects
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Neutrophils / drug effects
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Neutrophils / enzymology
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Neutrophils / metabolism
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Peptidylprolyl Isomerase
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Phosphorylation / drug effects
Substances
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Antibodies
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Antigens, CD
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Antigens, Neoplasm
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Antigens, Surface
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Avian Proteins
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BSG protein, human
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Blood Proteins
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Bsg protein, Gallus gallus
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Bsg protein, rat
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Membrane Glycoproteins
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Basigin
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cyclophilin B
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Mitogen-Activated Protein Kinases
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Cyclophilin A
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Cyclophilins
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Peptidylprolyl Isomerase