Abstract
A novel secreted cytokine, termed IL-17F, was cloned using nested RACE PCR. This cytokine bears homology to IL-17. IL-17F was expressed only in activated CD4(+) T cells and activated monocytes. Recombinant human IL-17F did not stimulate the proliferation of hematopoietic progenitors or the migration of mature leukocytes. However, it markedly inhibited the angiogenesis of human endothelial cells and induced endothelial cells to produce IL-2, TGF-beta, and monocyte chemoattractant protein-1.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Angiogenesis Inhibitors / biosynthesis
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Angiogenesis Inhibitors / genetics
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Angiogenesis Inhibitors / pharmacology*
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Base Sequence
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Cloning, Molecular
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Cytokines / biosynthesis
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Endothelium, Vascular / drug effects*
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Humans
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Interleukin-17 / genetics
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Interleukin-17 / pharmacology*
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Molecular Sequence Data
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Monocytes / metabolism*
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Neovascularization, Physiologic / drug effects*
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Polymerase Chain Reaction
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Recombinant Proteins / biosynthesis
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Sequence Homology, Amino Acid
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T-Lymphocytes / metabolism*
Substances
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Angiogenesis Inhibitors
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Cytokines
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IL17F protein, human
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Interleukin-17
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Recombinant Proteins