Abstract
Signaling lymphocyte activation molecule (SLAM), a 70-kDa costimulatory molecule that mediates CD28-independent proliferation of T cells and IFN-gamma production, has been identified on human T cells, immature thymocytes, and a subset of B cells. We have found that SLAM is expressed on mature but not immature dendritic cells (DC). However, the SLAM-associated protein, is missing in DC. SLAM surface expression is strongly up-regulated by IL-1beta. Addition of IL-1beta to the DC maturation mixture also increases the stimulatory properties of DC. These findings provide a new marker for DC maturation and help to explain two areas of DC biology. First, SLAM is a receptor for the measles virus, previously shown to infect DC. Second, SLAM could possibly contribute to the enhanced immunostimulatory functions of DC that are observed following the addition of IL-1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / pharmacology
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Antibodies, Monoclonal / pharmacology
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Antigens, CD
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CD40 Antigens / immunology
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CD83 Antigen
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Cell Differentiation / immunology
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Cell Membrane / immunology
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Cell Membrane / metabolism
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Cells, Cultured
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Dendritic Cells / cytology
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism*
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Epitopes, T-Lymphocyte / immunology
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Glycoproteins / analysis
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Glycoproteins / biosynthesis*
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Glycoproteins / genetics
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Glycoproteins / immunology
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Humans
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Immunoglobulins / analysis
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Immunoglobulins / biosynthesis*
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Immunoglobulins / genetics
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Immunoglobulins / immunology
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Intercellular Adhesion Molecule-1 / biosynthesis
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Interleukin-1 / pharmacology*
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Lymphocyte Activation*
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Lymphocyte Culture Test, Mixed
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Membrane Glycoproteins / biosynthesis*
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Peptides / immunology
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Protein Isoforms / analysis
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Protein Isoforms / genetics
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RNA, Messenger / analysis
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Receptors, Cell Surface
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Signal Transduction / immunology*
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Signaling Lymphocytic Activation Molecule Family Member 1
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T-Lymphocytes, Cytotoxic / immunology
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Up-Regulation / immunology*
Substances
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Adjuvants, Immunologic
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Antibodies, Monoclonal
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Antigens, CD
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CD40 Antigens
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Epitopes, T-Lymphocyte
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Glycoproteins
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Immunoglobulins
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Interleukin-1
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Membrane Glycoproteins
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Peptides
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Protein Isoforms
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RNA, Messenger
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Receptors, Cell Surface
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Intercellular Adhesion Molecule-1
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Signaling Lymphocytic Activation Molecule Family Member 1