Signaling lymphocytic activation molecule is expressed on mature CD83+ dendritic cells and is up-regulated by IL-1 beta

J Immunol. 2001 Aug 15;167(4):1989-95. doi: 10.4049/jimmunol.167.4.1989.

Abstract

Signaling lymphocyte activation molecule (SLAM), a 70-kDa costimulatory molecule that mediates CD28-independent proliferation of T cells and IFN-gamma production, has been identified on human T cells, immature thymocytes, and a subset of B cells. We have found that SLAM is expressed on mature but not immature dendritic cells (DC). However, the SLAM-associated protein, is missing in DC. SLAM surface expression is strongly up-regulated by IL-1beta. Addition of IL-1beta to the DC maturation mixture also increases the stimulatory properties of DC. These findings provide a new marker for DC maturation and help to explain two areas of DC biology. First, SLAM is a receptor for the measles virus, previously shown to infect DC. Second, SLAM could possibly contribute to the enhanced immunostimulatory functions of DC that are observed following the addition of IL-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology
  • Antibodies, Monoclonal / pharmacology
  • Antigens, CD
  • CD40 Antigens / immunology
  • CD83 Antigen
  • Cell Differentiation / immunology
  • Cell Membrane / immunology
  • Cell Membrane / metabolism
  • Cells, Cultured
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism*
  • Epitopes, T-Lymphocyte / immunology
  • Glycoproteins / analysis
  • Glycoproteins / biosynthesis*
  • Glycoproteins / genetics
  • Glycoproteins / immunology
  • Humans
  • Immunoglobulins / analysis
  • Immunoglobulins / biosynthesis*
  • Immunoglobulins / genetics
  • Immunoglobulins / immunology
  • Intercellular Adhesion Molecule-1 / biosynthesis
  • Interleukin-1 / pharmacology*
  • Lymphocyte Activation*
  • Lymphocyte Culture Test, Mixed
  • Membrane Glycoproteins / biosynthesis*
  • Peptides / immunology
  • Protein Isoforms / analysis
  • Protein Isoforms / genetics
  • RNA, Messenger / analysis
  • Receptors, Cell Surface
  • Signal Transduction / immunology*
  • Signaling Lymphocytic Activation Molecule Family Member 1
  • T-Lymphocytes, Cytotoxic / immunology
  • Up-Regulation / immunology*

Substances

  • Adjuvants, Immunologic
  • Antibodies, Monoclonal
  • Antigens, CD
  • CD40 Antigens
  • Epitopes, T-Lymphocyte
  • Glycoproteins
  • Immunoglobulins
  • Interleukin-1
  • Membrane Glycoproteins
  • Peptides
  • Protein Isoforms
  • RNA, Messenger
  • Receptors, Cell Surface
  • Intercellular Adhesion Molecule-1
  • Signaling Lymphocytic Activation Molecule Family Member 1