Abstract
BCL10 belongs to the caspase recruitment domain (CARD) family of proteins that regulate apoptosis and NF-kappaB signaling pathways. Analysis of BCL10-deficient mice has revealed that BCL10 mediates NF-kappaB activation by antigen receptors in B and T cells. We recently identified a subclass of CARD proteins (CARD9, CARD11, and CARD14) that may function to connect BCL10 to multiple upstream signaling pathways. We report here that CARD10 is a novel BCL10 interactor that belongs to the membrane-associated guanylate kinase family, a class of proteins that function to organize signaling complexes at plasma membranes. When expressed in cells, CARD10 binds to BCL10 and signals the activation of NF-kappaB through its N-terminal effector CARD domain. We propose that CARD10 functions as a molecular scaffold for the assembly of a BCL10 signaling complex that activates NF-kappaB.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Amino Acid Sequence
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Animals
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Apoptosis Regulatory Proteins
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B-Cell CLL-Lymphoma 10 Protein
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Binding Sites
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CARD Signaling Adaptor Proteins
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Cell Line
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Cell Membrane / metabolism
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Genes, Reporter
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Guanylate Kinases
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Humans
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Mammals
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Molecular Sequence Data
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NF-kappa B / metabolism*
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Neoplasm Proteins / metabolism*
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Nucleoside-Phosphate Kinase / chemistry
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Nucleoside-Phosphate Kinase / genetics*
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Nucleoside-Phosphate Kinase / metabolism*
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Organ Specificity
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Sequence Alignment
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Sequence Homology, Amino Acid
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Signal Transduction
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Transcription, Genetic
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Transfection
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src Homology Domains
Substances
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Adaptor Proteins, Signal Transducing
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Apoptosis Regulatory Proteins
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B-Cell CLL-Lymphoma 10 Protein
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BCL10 protein, human
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CARD Signaling Adaptor Proteins
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CARD10 protein, human
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Card10 protein, mouse
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NF-kappa B
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Neoplasm Proteins
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Nucleoside-Phosphate Kinase
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Guanylate Kinases