Molecular cloning and functional characterization of a human scavenger receptor with C-type lectin (SRCL), a novel member of a scavenger receptor family

Biochem Biophys Res Commun. 2001 Feb 2;280(4):1028-35. doi: 10.1006/bbrc.2000.4210.

Abstract

Using a human placenta cDNA library, we cloned a novel member belonging to the scavenger receptor family. Complementary DNA of this clone encodes a type II transmembranous glycoprotein containing a collagen-like domain, which are typical structural characteristics of scavenger receptor class A. This protein also contains a C-type lectin/carbohydrate recognition domain (C-type CRD) located at the C-terminus. We designated this as Scavenger Receptor with C-type Lectin (SRCL) type I. We also isolated human SRCL type II, which lacks the C-type CRD. Northern blot analysis revealed that hSRCL type I and type II mRNAs are abundantly expressed in adult human tissues. When hSRCL type I and type II were expressed in CHO-K1 cells, they were localized in the plasma membrane forming clusters on the surface. Ligand-binding studies of CHO-K1 cells expressing hSRCL type I and type II demonstrated their specific binding capacity in Escherichia coli and Staphylococcus aureus. These results indicate that hSRCL is a novel bacteria-binding receptor containing a C-type CRD and this receptor may play an important role in host defense.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • CHO Cells
  • Cell Membrane / metabolism
  • Chromosome Mapping
  • Chromosomes, Human, Pair 18
  • Cloning, Molecular
  • Collagen / chemistry
  • Collectins*
  • Cricetinae
  • DNA, Complementary / metabolism
  • Escherichia coli / metabolism
  • Gene Library
  • Glycoproteins / chemistry
  • Humans
  • Immunohistochemistry
  • Ligands
  • Membrane Proteins*
  • Microscopy, Fluorescence
  • Models, Biological
  • Molecular Sequence Data
  • Placenta / metabolism
  • Precipitin Tests
  • Protein Binding
  • Protein Structure, Tertiary
  • Receptors, Immunologic / biosynthesis
  • Receptors, Immunologic / chemistry*
  • Receptors, Immunologic / genetics*
  • Receptors, Lipoprotein*
  • Receptors, Scavenger
  • Scavenger Receptors, Class A
  • Scavenger Receptors, Class B
  • Sequence Homology, Amino Acid
  • Staphylococcus aureus / metabolism
  • Tissue Distribution

Substances

  • COLEC12 protein, human
  • Collectins
  • DNA, Complementary
  • Glycoproteins
  • Ligands
  • Membrane Proteins
  • Receptors, Immunologic
  • Receptors, Lipoprotein
  • Receptors, Scavenger
  • Scarb1 protein, mouse
  • Scavenger Receptors, Class A
  • Scavenger Receptors, Class B
  • Collagen

Associated data

  • GENBANK/AB038518
  • GENBANK/AB052103