Abstract
Using a human placenta cDNA library, we cloned a novel member belonging to the scavenger receptor family. Complementary DNA of this clone encodes a type II transmembranous glycoprotein containing a collagen-like domain, which are typical structural characteristics of scavenger receptor class A. This protein also contains a C-type lectin/carbohydrate recognition domain (C-type CRD) located at the C-terminus. We designated this as Scavenger Receptor with C-type Lectin (SRCL) type I. We also isolated human SRCL type II, which lacks the C-type CRD. Northern blot analysis revealed that hSRCL type I and type II mRNAs are abundantly expressed in adult human tissues. When hSRCL type I and type II were expressed in CHO-K1 cells, they were localized in the plasma membrane forming clusters on the surface. Ligand-binding studies of CHO-K1 cells expressing hSRCL type I and type II demonstrated their specific binding capacity in Escherichia coli and Staphylococcus aureus. These results indicate that hSRCL is a novel bacteria-binding receptor containing a C-type CRD and this receptor may play an important role in host defense.
Copyright 2001 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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CHO Cells
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Cell Membrane / metabolism
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Chromosome Mapping
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Chromosomes, Human, Pair 18
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Cloning, Molecular
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Collagen / chemistry
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Collectins*
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Cricetinae
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DNA, Complementary / metabolism
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Escherichia coli / metabolism
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Gene Library
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Glycoproteins / chemistry
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Humans
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Immunohistochemistry
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Ligands
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Membrane Proteins*
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Microscopy, Fluorescence
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Models, Biological
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Molecular Sequence Data
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Placenta / metabolism
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Precipitin Tests
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Protein Binding
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Protein Structure, Tertiary
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Receptors, Immunologic / biosynthesis
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Receptors, Immunologic / chemistry*
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Receptors, Immunologic / genetics*
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Receptors, Lipoprotein*
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Receptors, Scavenger
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Scavenger Receptors, Class A
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Scavenger Receptors, Class B
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Sequence Homology, Amino Acid
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Staphylococcus aureus / metabolism
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Tissue Distribution
Substances
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COLEC12 protein, human
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Collectins
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DNA, Complementary
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Glycoproteins
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Ligands
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Membrane Proteins
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Receptors, Immunologic
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Receptors, Lipoprotein
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Receptors, Scavenger
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Scarb1 protein, mouse
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Scavenger Receptors, Class A
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Scavenger Receptors, Class B
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Collagen
Associated data
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GENBANK/AB038518
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GENBANK/AB052103