Cloning and characterization of AWP1, a novel protein that associates with serine/threonine kinase PRK1 in vivo

Gene. 2000 Oct 3;256(1-2):113-21. doi: 10.1016/s0378-1119(00)00365-6.

Abstract

We describe the cloning and expression of cDNAs encoding a novel human protein of 208 amino acid residues with a predicted molecular mass of 22.6kDa and its mouse homologue. We name this protein as AWP1 (associated with PRK1). AWP1 is a ubiquitously expressed protein, and the Awp1 gene is switched on during early human and mouse development. When expressed in COS-1 cells, the Myc-tagged AWP1 has an apparent molecular mass higher than that deduced from its amino acid sequence. AWP1 possesses a conserved zf-A20 zinc finger domain at its N-terminal and a zf-AN1 zinc finger domain at its C-terminal. Co-immunoprecipitation experiments revealed that mouse AWP1 specifically interacts with a rat serine/threonine protein kinase PRK1 in vivo. Hence, AWP1 may play a regulatory role in mammalian signal transduction pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • COS Cells
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Chromosome Mapping
  • Chromosomes, Human, Pair 15 / genetics
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Escherichia coli / genetics
  • Female
  • Gene Expression
  • Glutathione Transferase / genetics
  • Glutathione Transferase / metabolism
  • Humans
  • Mice
  • Molecular Sequence Data
  • Plant Proteins*
  • Protein Binding
  • Protein Serine-Threonine Kinases
  • Proteins / genetics*
  • Proteins / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Receptor Protein-Tyrosine Kinases / genetics
  • Receptor Protein-Tyrosine Kinases / metabolism*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Zinc Fingers

Substances

  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • DNA, Complementary
  • Plant Proteins
  • Proteins
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • ZFAND6 protein, human
  • Zfand6 protein, mouse
  • Glutathione Transferase
  • PRK1 protein, Petunia inflata
  • Receptor Protein-Tyrosine Kinases
  • Protein Serine-Threonine Kinases

Associated data

  • GENBANK/AJ251095
  • GENBANK/AJ251508