Abstract
We describe the cloning and expression of cDNAs encoding a novel human protein of 208 amino acid residues with a predicted molecular mass of 22.6kDa and its mouse homologue. We name this protein as AWP1 (associated with PRK1). AWP1 is a ubiquitously expressed protein, and the Awp1 gene is switched on during early human and mouse development. When expressed in COS-1 cells, the Myc-tagged AWP1 has an apparent molecular mass higher than that deduced from its amino acid sequence. AWP1 possesses a conserved zf-A20 zinc finger domain at its N-terminal and a zf-AN1 zinc finger domain at its C-terminal. Co-immunoprecipitation experiments revealed that mouse AWP1 specifically interacts with a rat serine/threonine protein kinase PRK1 in vivo. Hence, AWP1 may play a regulatory role in mammalian signal transduction pathways.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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COS Cells
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Chromosome Mapping
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Chromosomes, Human, Pair 15 / genetics
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Escherichia coli / genetics
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Female
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Gene Expression
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Glutathione Transferase / genetics
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Glutathione Transferase / metabolism
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Humans
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Mice
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Molecular Sequence Data
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Plant Proteins*
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Protein Binding
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Protein Serine-Threonine Kinases
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Proteins / genetics*
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Proteins / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Receptor Protein-Tyrosine Kinases / genetics
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Receptor Protein-Tyrosine Kinases / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Tissue Distribution
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Zinc Fingers
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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DNA, Complementary
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Plant Proteins
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Proteins
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RNA, Messenger
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Recombinant Fusion Proteins
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ZFAND6 protein, human
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Zfand6 protein, mouse
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Glutathione Transferase
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PRK1 protein, Petunia inflata
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Receptor Protein-Tyrosine Kinases
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Protein Serine-Threonine Kinases
Associated data
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GENBANK/AJ251095
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GENBANK/AJ251508