Abstract
Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. The yeast Saccharomyces cerevisiae Rad18 protein plays a pivotal role in the process together with the Rad6 protein. Here, we have cloned a human homologue of RAD18, hRAD18. It maps on chromosome 3p24-25, where deletions are often found in lung, breast, ovary, and testis cancers. In vivo, hRad18 protein binds to hHR6 protein through a conserved ring-finger motif. Stable transformants with hRad18 mutated in this motif become sensitive to UV, methyl methanesulfonate, and mitomycin C, and are defective in the replication of UV-damaged DNA. Thus, hRAD18 is a functional homologue of RAD18.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Chromosome Mapping
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Chromosomes, Human, Pair 3
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DNA Repair*
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DNA Replication*
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DNA-Binding Proteins / metabolism*
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Expressed Sequence Tags
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Gene Library
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Humans
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Ligases / metabolism
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Methyl Methanesulfonate / pharmacology
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Mitomycin / pharmacology
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Molecular Sequence Data
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Mutagens / pharmacology*
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Polymerase Chain Reaction
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Protein Binding
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
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Two-Hybrid System Techniques
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Ubiquitin-Conjugating Enzymes
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Ultraviolet Rays / adverse effects
Substances
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DNA-Binding Proteins
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Mutagens
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RAD18 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Mitomycin
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Methyl Methanesulfonate
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Ubiquitin-Conjugating Enzymes
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Ligases