Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R

M H Xie; S Aggarwal; W H Ho; J Foster; Z Zhang; J Stinson; W I Wood; A D Goddard; A L Gurney

doi:10.1074/jbc.M005304200

Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R

J Biol Chem. 2000 Oct 6;275(40):31335-9. doi: 10.1074/jbc.M005304200.

Authors

M H Xie¹, S Aggarwal, W H Ho, J Foster, Z Zhang, J Stinson, W I Wood, A D Goddard, A L Gurney

Affiliation

¹ Department of Molecular Biology, Genentech, Inc., South San Francisco, California 94080, USA.

PMID: 10875937
DOI: 10.1074/jbc.M005304200

Abstract

We report the identification of a novel human cytokine, distantly related to interleukin (IL)-10, which we term IL-22. IL-22 is produced by activated T cells. IL-22 is a ligand for CRF2-4, a member of the class II cytokine receptor family. No high affinity ligand has yet been reported for this receptor, although it has been reported to serve as a second component in IL-10 signaling. A new member of the interferon receptor family, which we term IL-22R, functions as a second component together with CRF2-4 to enable IL-22 signaling. IL-22 does not bind the IL-10R. Cell lines were identified that respond to IL-22 by activation of STATs 1, 3, and 5, but were unresponsive to IL-10. In contrast to IL-10, IL-22 does not inhibit the production of proinflammatory cytokines by monocytes in response to LPS nor does it impact IL-10 function on monocytes, but it has modest inhibitory effects on IL-4 production from Th2 T cells.

MeSH terms

Amino Acid Sequence
Blotting, Western
Cell Line
DNA-Binding Proteins / metabolism
Electrophoresis, Polyacrylamide Gel
Enzyme Activation
Enzyme-Linked Immunosorbent Assay
Expressed Sequence Tags
Flow Cytometry
Humans
Interleukin-10 / metabolism
Interleukin-10 Receptor beta Subunit
Interleukin-22
Interleukins / chemistry*
Interleukins / metabolism*
Ligands
Lipopolysaccharides / pharmacology
Membrane Glycoproteins*
Molecular Sequence Data
Monocytes / metabolism
Protein Binding
Receptors, Cytokine / metabolism*
Receptors, Interleukin / metabolism*
Receptors, Interleukin-10
Sequence Homology, Amino Acid
Signal Transduction*
T-Lymphocytes / metabolism
Th2 Cells / metabolism
Tumor Necrosis Factor-alpha / metabolism

Substances

DNA-Binding Proteins
IL10RB protein, human
Interleukin-10 Receptor beta Subunit
Interleukins
Ligands
Lipopolysaccharides
Membrane Glycoproteins
Receptors, Cytokine
Receptors, Interleukin
Receptors, Interleukin-10
Tumor Necrosis Factor-alpha
interleukin-22 receptor
Interleukin-10

Associated data

GENBANK/AF279437
GENBANK/AF286095