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Binding affinity to human full length recombinant His-tagged HuR expressed in Escherichia coli Rosetta DH5alpha assessed as inhibition of interaction with single-strand Bi-AU RNA probe by AlphaScreen assay
Assay data:8 Active, 17 Activity ≤ 1 µM, 17 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to human full length recombinant HuR expressed in Escherichia coli Rosetta DH5alpha measured for 30 mins by DMR analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Antimigratory activity in human MDA-MB-231 cells at 5 to 20 uM after 24 to 48 hrs by scratch assay
SummaryCompounds, ActivePubMed Citation
Antimigratory activity in human PANC1 cells at 5 to 20 uM after 24 to 48 hrs by scratch assay
Stabilization of closed conformation of human full length recombinant HuR RRM1-RRM2 domain at 0.6 equiv by 2D 1H 15N HSQC spectra analysis
Inhibition of HuR-VEGF mRNA interaction in human MCF7 cells at 5 uM after 6 hrs by qRT-PCR method
Inhibition of HuR-CTNNB1 mRNA interaction in human MCF7 cells at 5 uM after 6 hrs by qRT-PCR method
Inhibition of HuR-ERBB2 mRNA interaction in human MCF7 cells at 5 uM after 6 hrs by qRT-PCR method
Inhibition of HuR-VEGF mRNA interaction in human MCF7 cells at 5 uM after 6 hrs by RNA immunoprecipitation assay
Inhibition of HuR-CTNNB1 mRNA interaction in human MCF7 cells at 5 uM after 6 hrs by RNA immunoprecipitation assay
Inhibition of HuR-ERBB2 mRNA interaction in human MCF7 cells at 5 uM after 6 hrs by RNA immunoprecipitation assay
Binding affinity to human recombinant HuR RRM1-2 domain expressed in Escherichia coli Rosetta DH5alpha assessed as inhibition of interaction with 5'-DY681-labeled AU-rich RNA probe at 0.3 to 10 uM after 30 mins by REMSA
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