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CAMK2D SGC Tm panel (DSF)
Assay data:1 Tested
SummaryRelated BioAssays by Target
CAMK2B SGC Tm panel (DSF)
Inhibition of CAMK2B (unknown origin) at 10 uM by biochemical hotspot kinase assay relative to control
Inhibition of CAMK2alpha (unknown origin) at 1000 nM relative to control
Inhibition of CAMK2alpha (unknown origin)
Assay data:1 Activity ≤ 1 µM, 1 Tested
Selectivity interaction (Upstate Kinase Profiler service (Millipore)) EUB0000056c CAMK2B
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Thermal Shift Assay. Domain: start/stop: T11-N335
Assay data:27 Tested
Thermal Shift Assay. Domain: start/stop: T8-Q666
Inhibition of [3H]NCS-382 binding to CaMK2alpha in rat brain cerebral cortex membrane homogenates at 100 uM measured after 60 mins by TopCount scintillation counting method relative to control
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of [3H]NCS-382 binding to CaMK2alpha (unknown origin)
Assay data:4 Active, 1 Activity ≤ 1 µM, 6 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of [3H]NCS-382 binding to CaMK2alpha in rat cerebrocortical membranes
Assay data:2 Active, 1 Activity ≤ 1 nM, 2 Activity ≤ 1 µM, 2 Tested
Binding affinity to recombinant human CaMK2alpha (345 to 475 residues) expressed in Escherichia coli BL21 DE3 assessed as thermal stability by measuring change in melting temperature at 0.625 to 160 uM by SYPRO orange dye based differential scanning fluorimetry
Binding affinity to recombinant human CaMK2alpha (345 to 475 residues) expressed in Escherichia coli BL21 DE3 assessed as thermal stability by measuring change in melting temperature at 160 uM by SYPRO orange dye based differential scanning fluorimetry
Binding affinity to recombinant human CaMK2alpha Phe467Met mutant expressed in Escherichia coli BL21 DE3 assessed as dissociation constant by surface plasmon resonance assay
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
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