Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Stabilization of mono-ubiquitinated UBE2E1 (unknown origin) at 500 uM assessed as decrease in poly-Ub measured by poly-ubiquitination assay
Assay data:3 Tested
SummaryRelated BioAssays by Target
HTRF Assay from US Patent US9663525: "Pyrazolopyrimidinyl inhibitors of ubiquitin-activating enzyme"
Assay data:119 Active, 119 Activity ≤ 1 µM, 119 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Binding affinity to human recombinant UbcH5c assessed as dissociation rate constant by SPR analysis
Assay data:4 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to human recombinant UbcH5c assessed as association rate constant by SPR analysis
Inhibition of UbcH5c in mouse b.End3 cells assessed as decrease in TNF-alpha-mediated IkB-alpha degradation at 10 uM preincubated for 2 hrs followed by TNF-alpha stimulation for 20 mins by Western blot analysis
Assay data:10 Active, 10 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of UbcH5c in mouse b.End3 cells assessed as decrease in TNF-alpha-mediated IkB-alpha phosphorylation at 10 uM preincubated for 2 hrs followed by TNF-alpha stimulation for 20 mins by Western blot analysis
Binding affinity to trypsin digested recombinant UbcH5c (unknown origin) assessed as covalent adduct formation with Cys111 residues after 1 hr by LC/MS/MS analysis
Assay data:1 Tested
Binding affinity to trypsin digested recombinant UbcH5c (unknown origin) assessed as covalent adduct formation with Cys85 residues after 1 hr by LC/MS/MS analysis
Assay data:1 Active, 1 Tested
Binding affinity to human recombinant UbcH5c by SPR analysis
Assay data:5 Active, 2 Activity ≤ 1 µM, 5 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of UbcH5b (unknown origin) at 2.5 to 10 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis
Inhibition of UbcH5a (unknown origin) at 2.5 to 10 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis
Inhibition of UbcH5c (unknown origin) preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis
Assay data:5 Tested
Inhibition of UbcH5c in mouse b.End3 cells assessed as decrease in TNF-alpha-mediated p65 phosphorylation preincubated for 2 hrs followed by TNF-alpha stimulation for 20 mins by Western blot analysis
Assay data:6 Tested
Inhibition of UbcH5c in mouse b.End3 cells assessed as decrease in TNF-alpha-mediated IkB-alpha degradation preincubated for 2 hrs followed by TNF-alpha stimulation for 20 mins by Western blot analysis
Inhibition of UbcH5c in mouse b.End3 cells assessed as decrease in TNF-alpha-mediated IkB-alpha phosphorylation preincubated for 2 hrs followed by TNF-alpha stimulation for 20 mins by Western blot analysis
Inhibition of UbcH5c (unknown origin) at 2.5 to 10 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis
Inhibition of UbcH5c (unknown origin) at 1 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis
Inhibition of UbcH5c (unknown origin) at 5 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis
Assay data:17 Active, 52 Tested
Inhibition of UbcH5c (unknown origin) at 10 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis
Assay data:11 Active, 15 Tested
Binding affinity to UbcH5c in human HeLa cells up to 40 uM after 40 mins by Azide-Fluor 488 dye-based fluorescence imaging assay
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on