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Inhibition of USP5 (unknown origin) at 10 uM relative to control
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human 6His-tagged USP5 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis
Assay data:1 Tested
Inhibition of Usp5 in human MM1S cells at 0.625 to 2.5 uM
Assay data:1 Active, 1 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
Inhibition of Usp5 in Vemurafenib-resistant human A375 cells assessed as increase in p53 expression
Inhibition of Usp5 in Vemurafenib-sensitive human A375 cells assessed as increase in p53 expression
Inhibition of Usp5 in Vemurafenib-resistant human A375 cells
Assay data:1 Active, 2 Tested
Inhibition of Usp5 in Vemurafenib-sensitive human A375 cells
Assay data:2 Active, 2 Tested
Inhibition of Usp5 in human A375 cells assessed as reduction in DUB activity by HA blotting
Inhibition of Usp5 in human SK-MEL-28 cells assessed as reduction in DUB activity by HA blotting
Inhibition of N-terminal Avitag-fused His6-tagged USP5 (unknown origin) (1 to 835 residues) labelled with 5-fluorotryptophan expressed in BL21 (DE3) Codon Plus RIL Escherichia coli using ubiquitin-rhodamine 110 as substrate and measured for 10 mind by fluorescence based assay relative to control
Inhibition of N-terminal Avitag-fused His6-tagged USP5 (unknown origin) (1 to 835 residues) labelled with 5-fluorotryptophan expressed in BL21 (DE3) Codon Plus RIL Escherichia coli using ubiquitin-rhodamine 110 as substrate and measured for 10 mind by fluorescence based assay
Binding affinity to full length N-terminal Avitag-fused His6-tagged USP5 R221A mutant (unknown origin) (1 to 835 residues) labelled with 5-fluorotryptophan expressed in BL21 (DE3) Codon Plus RIL Escherichia coli incubated for 35 to 60 secs by SPR analysis
Binding affinity to full length N-terminal Avitag-fused His6-tagged USP5 (unknown origin) (1 to 835 residues) labelled with 5-fluorotryptophan expressed in BL21 (DE3) Codon Plus RIL Escherichia coli incubated for 35 to 60 secs by SPR analysis
Binding affinity to N-terminal Avitag-fused His6-tagged USP5 ZnF-UBD (unknown origin) (171 to 290 residues) labelled with 5-fluorotryptophan expressed in BL21 (DE3) Codon Plus RIL Escherichia coli incubated for 35 to 60 secs by SPR analysis
Assay data:38 Tested
Binding affinity to N-terminal Avitag-fused His6-tagged USP5 ZnF-UBD (unknown origin) (171 to 290 residues) labelled with 5-fluorotryptophan expressed in BL21 (DE3) Codon Plus RIL Escherichia coli at 25:1 compound to protein ratio by 19F NMR spectra analysis
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of human recombinant isopeptidase T assessed as reduction in cleavage of luminescent substrate Ub-AML at 100 uM
Binding affinity to USP5 in human A549 cells lysates grown on SILAC media at 10 uM incubated for 1 hr by LC-MS/MS analysis relative to untreated control
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Inhibition of USP5 (unknown origin) using Ub-AMC as substrate preincubated for 30 mins followed by substrate addition measured after 60 mins by fluorescence based assay
Binding affinity to USP5 in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay
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