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Binding affinity to PGAM1 (unknown origin) assessed as dissociation constant by fluorescence-based assay
Assay data:1 Active, 1 Tested
SummaryRelated BioAssays by Target
Inhibition of His-tagged recombinant PGAM1 (unknown origin) assessed as reduction in ATP production in presence of pyruvate kinase M1 and ATP by Kinase-Glo Max luminescent kinase assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Inhibition of His-tagged recombinant PGAM1 (unknown origin) expressed in Escherichia coli BL21 assessed as decrease in absorbance from oxidation of NADH in presence of LDH, pyruvate kinase M1 and NADH by microplate reader assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Allosteric inhibition of PGAM1 (unknown origin) assessed as decrease in absorbance in presence of LDH, pyruvate kinase M2 and NADH by spectroscopic analysis
Inhibition of recombinant PGAM1 (unknown origin) assessed as decrease in absorbance in presence of LDH, pyruvate kinase M2 and NADH by spectroscopic analysis
Assay data:4 Active, 3 Activity ≤ 1 µM, 4 Tested
Inhibition of His6-tagged human PGAM1 expressed in Escherichia coli BL21(DE3)pLysS cells assessed as decrease in autofluorescence from oxidation of NADH in presence of LDH and pyruvate kinase M1 by multiple enzymes coupled assay
Assay data:2 Tested
Inhibition of human PGAM1 in human MDA-MB-231 cells assessed as decrease in PGAM1 activity incubated for 2 hrs by UV/Vis spectrophotometry
Assay data:1 Tested
Inhibition of PGAM1 in human MDA-MB-231 cells
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of PGAM1 (unknown origin)
Assay data:9 Active, 1 Activity ≤ 1 µM, 21 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of recombinant PGAM1 (unknown origin) using 3-PG as substrate incubated for 2 mins in presence of ADP by microplate reader assay
Inhibition of recombinant His-tagged PGAM1 (unknown origin) expressed in Escherichia coli BL21 using 3-PG as substrate in presence of ADP by Kinase-Glo max luminescent assay
Inhibition of human recombinant PGAM1 by measuring decrease in OD by enzyme based assay
Assay data:22 Active, 13 Activity ≤ 1 µM, 25 Tested
Inhibition of PGAM1 in primary leukemia cells derived from AML patient assessed as reduction in lactate levels at 20 uM in presence of 5 uM methyl-2-PG by spectrophotometry
Inhibition of PGAM1 in primary leukemia cells derived from AML patient assessed as reduction in lactate levels at 20 uM by spectrophotometry
SummaryCompounds, ActiveRelated BioAssays by Target
Inhibition of PGAM1 in primary leukemia cells derived from AML patient at 20 uM in presence of 3-PG substrate by fluorescence based enolase/ pyruvate kinase M/LDH coupled enzyme assay
Inhibition of PGAM1 in primary leukemia cells derived from AML patient assessed as reduction in 2-PG levels at 20 uM by spectrophotometry
Inhibition of PGAM1 in primary leukemia cells derived from AML patient assessed as increase in 3-PG levels at 20 uM by spectrophotometry
Inhibition of PGAM1 in human NCI-H1299 cells assessed as decrease in RNA biosynthesis at 20 uM in presence of D-[6-14C]-glucose incubated for 2 hrs by scintillation counting method
Inhibition of PGAM1 in human NCI-H1299 cells assessed as decrease in lipid biosynthesis at 20 uM in presence of D-[6-14C]-glucose incubated for 2 hrs by scintillation counting method
Inhibition of PGAM1 in human NCI-H1299 cells assessed as decrease in NADPH/NADP+ ratio at 20 uM by spectrophotometry
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