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Binding affinity to PRDX1 in human DOHH-2 cells by MS analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to PRDX1 in human MDA-MB-453 cells at 10 uM measured after 6 hrs in presence of 100 uM PES by pull-down assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to PRDX1 in human DOHH-2 cells at 10 uM measured after 6 hrs in presence of 100 uM PES by pull-down assay
Binding affinity to PRDX1 in human DOHH-2 cells measured after 6 hrs by pull-down assay
Binding affinity to PRDX1 in human MDA-MB-453 cells measured after 6 hrs by pull-down assay
Binding affinity to peroxiredoxin-1 (unknown origin) assessed as change in enthalpy by isothermal titration calorimetry
Binding affinity to peroxiredoxin-1 (unknown origin) assessed as association constant by isothermal titration calorimetry
Inhibition of PRDX1 in human PMA-differentiated LPS-stimulated THP1 cells assessed as reduction in NLRP3 inflammasome activation preincubated for 1 hr followed by addition of ATP and mesured after 1 hr by ELISA
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to full-length human PRDX1 by surface plasmon resonance analysis
Assay data:2 Active, 2 Tested
Induction of PRDX1 nuclear translocation in human ACHN cells at 25 to 50 nM measured after 24 hrs by DAPI staining-based microscopic analysis
Activation of PRDX1 in human ACHN cells assessed as reduction in reactive oxygen species level at 25 to 50 nM measured after 24 hrs by Cell-rox reagent-based flow cytometry
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Binding affinity to peroxiredoxin-1 in human Hela cells lysates assessed as protein enrichment by measuring normalized heavy/light ratio at by nano-LC-ESIMS/MS analysis
Binding affinity to recombinant human N-terminal 6His-tagged peroxiredoxin-1 C173A mutant expressed in Escherichia coli BL21 after 1 hr by LC-MS/MS analysis
Binding affinity to wild type recombinant human N-terminal 6His-tagged peroxiredoxin-1 expressed in Escherichia coli BL21 up to 200 uM by immunoblot analysis
Binding affinity to wild type recombinant human N-terminal 6His-tagged peroxiredoxin-1 expressed in Escherichia coli BL21 at 0.625 to 40 uM by immunoblot analysis
Assay data:1 Active, 2 Tested
Binding affinity to peroxiredoxin-1 in human HeLa cell lysate incubated overnight by LC-MS/MS based pull down assay
Binding affinity to peroxiredoxin-1 in human Ramos cell lysate incubated overnight by LC-MS/MS based pull down assay
Binding affinity to peroxiredoxin-1 in human HeLa cell lysate incubated overnight by SDS-PAGE based pull down assay
Binding affinity to peroxiredoxin-1 in human Ramos cell lysate incubated overnight by SDS-PAGE based pull down assay
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