Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Uncompetitive inhibition of human NNMT assessed as Km of SAM
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Uncompetitive inhibition of human NNMT assessed as Km of nicotinamide
Inhibition of human NNMT
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of human NNMT in human K562 cells measured after 24 hrs by quantitative mass spectrometry
Assay data:7 Active, 7 Activity ≤ 1 µM, 7 Tested
Inhibition of human NNMT measured after 20 mins by luminescence based methyltransferase assay
Assay data:15 Active, 10 Activity ≤ 1 µM, 44 Tested
Inhibition of N-terminal 6His-tagged full length human recombinant NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) cells using S-adenosyl-L-methionine and NAM as substrate incubated for 2 hrs by by RapidFire Mass Spectrometry
Assay data:1 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of NNMT in HEK293 cells measured after 20 to 24 hrs by RapidFire Mass Spectrometry
Assay data:2 Active, 1 Activity ≤ 1 µM, 6 Tested
Inhibition of N-terminal 6His-tagged full length human recombinant NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) cells using S-adenosyl-L-methionine and NAM as substrate incubated for 22 to 24 hrs by RapidFire Mass Spectrometry
Assay data:8 Active, 7 Activity ≤ 1 µM, 14 Tested
Inhibition of NNMT (unknown origin) measured at 100 uM incubated for 10 mins in presence of nicotinamide by fluorescence-based SAHH-coupled assay
Binding affinity to human NNMT assessed as change in entropy by isothermal titration calorimetry
Binding affinity to human NNMT assessed as change in enthalpy by isothermal titration calorimetry
Binding affinity to human NNMT assessed as dissociation constant by isothermal titration calorimetry
Inhibition of recombinant NNMT (unknown origin) assessed as reduction in MNA concentration incubated for 10 mins measured after 30 mins by LC-MS/MS-MRM analysis
Assay data:32 Active, 16 Activity ≤ 1 µM, 60 Tested
Inhibition of recombinant NNMT (unknown origin) assessed as reduction in MNA concentration incubated for 10 mins by UHP-HILIC-MS analysis
Binding affinity to human NNMT expressed in Escherichia coli BL21(DE3) assessed as gibbs free energy change
Assay data:3 Tested
NNMT Enzymatic Activity Assay from Article 10.1021/acs.biochem.6b00733: "A Rapid and Efficient Assay for the Characterization of Substrates and Inhibitors of Nicotinamide N-Methyltransferase."
Assay data:1 Active, 7 Tested
Inhibition of human recombinant NNMT using nicotinamide and SAM as substrate assessed as reduction in 1-methyl-nicotinamide formation incubated for 2 hrs by RapidFire Mass spectroscopy
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Ratio of IC50 for inhibition of human NNMT expressed in HEK293 cells to IC50 for inhibition of human recombinant NNMT
Assay data:2 Tested
Inhibition of human NNMT expressed in HEK293 cells assessed as reduction in 1-methyl-nicotinamide formation measured after 24 hrs by RapidFire Mass spectroscopy
Assay data:2 Active, 1 Activity ≤ 1 µM, 4 Tested
Inhibition of human recombinant NNMT using nicotinamide and SAM as substrate assessed as reduction in 1-methyl-nicotinamide formation incubated for 22 to 24 hrs by RapidFire Mass spectroscopy
Assay data:24 Active, 4 Activity ≤ 1 nM, 22 Activity ≤ 1 µM, 27 Tested
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on