Alternative titles; symbols
HGNC Approved Gene Symbol: INTS2
Cytogenetic location: 17q23.2 Genomic coordinates (GRCh38): 17:61,865,367-61,927,982 (from NCBI)
INTS2 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; 180680) and U2 (RNU2; 180690) (Baillat et al., 2005).
By sequencing clones obtained from a size-fractionated adult brain cDNA library, Nagase et al. (1999) cloned INTS2, which they designated KIAA1287. The predicted protein has 1,209 amino acids, and the 5-prime and 3-prime untranslated regions contain SINEs, including Alu and MIR sequences. RT-PCR ELISA detected moderate expression in whole brain, testis, and ovary, low expression in lung and liver, and no expression in heart, pancreas, kidney, and spleen. Caudate nucleus and corpus callosum showed moderate to low expression, with little to no expression in other brain regions examined.
By SDS-PAGE of proteins that copurified with INTS11 (CPSF3L; 611354) in HEK293 cells, Baillat et al. (2005) identified INTS2 as a subunit of the Integrator complex.
By radiation hybrid analysis, Nagase et al. (1999) mapped the INTS2 gene to chromosome 17.
Baillat, D., Hakimi, M.-A., Naar, A. M., Shilatifard, A., Cooch, N., Shiekhattar, R. Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II. Cell 123: 265-276, 2005. [PubMed: 16239144] [Full Text: https://doi.org/10.1016/j.cell.2005.08.019]
Nagase, T., Ishikawa, K., Kikuno, R., Hirosawa, M., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 6: 337-345, 1999. [PubMed: 10574462] [Full Text: https://doi.org/10.1093/dnares/6.5.337]