HGNC Approved Gene Symbol: ING5
Cytogenetic location: 2q37.3 Genomic coordinates (GRCh38): 2:241,687,020-241,729,478 (from NCBI)
By searching for sequences similar to ING1 (601566) and ING2 (604215), followed by PCR and RACE of a placenta cDNA library, Shiseki et al. (2003) cloned ING5, which they called p28ING5. The deduced 240-amino acid protein contains a C-terminal plant homeodomain finger motif and 2 nuclear localization signals.
Shiseki et al. (2003) showed that overexpression of ING4 (608524) or ING5 in cancer cell lines diminished colony-forming efficiency, decreased cell population in S phase, and induced p53 (191170)-dependent apoptosis. Both ING4 and ING5 activated the WAF1 (116899) promoter and induced WAF1 expression. ING4 and ING5 physically interacted with p300 (602700), a member of histone acetyltransferase complexes, and with p53 in vivo, and they enhanced acetylation of p53 at lys382.
By immunopurifying HeLa cell proteins that associated with ING5, Doyon et al. (2006) found that ING5 was associated with 2 different histone acetyltransferase complexes, one containing HBO1 (MYST2; 609880) and the other containing MOZ (MYST3; 601408) and MORF (MYST4; 605880). In vitro acetyltransferase assays indicated that the histone specificity of the 2 complexes differed, with the ING5-HBO1 complex targeting histone H4 (see 602822) and the ING5-MOZ-MORF complex targeting H3 (see 602810). Knockdown of ING5 with small interfering RNA resulted in cells unable to complete S phase. ING5-depleted HBO1 complexes were defective in their ability to acetylate nucleosomal histones.
The International Radiation Hybrid Mapping Consortium mapped the ING5 gene to chromosome 2 (SHGC-64331).
Doyon, Y., Cayrou, C., Ullah, M., Landry, A.-J., Cote, V., Selleck, W., Lane, W. S., Tan, S., Yang, X.-J., Cote, J. ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation. Molec. Cell 21: 51-64, 2006. [PubMed: 16387653] [Full Text: https://doi.org/10.1016/j.molcel.2005.12.007]
Shiseki, M., Nagashima, M., Pedeux, R. M., Kitahama-Shiseki, M., Miura, K., Okamura, S., Onogi, H., Higashimoto, Y., Appella, E., Yokota, J., Harris, C. C. p29ING4 and p28ING5 bind to p53 and p300, and enhance p53 activity. Cancer Res. 63: 2373-2378, 2003. [PubMed: 12750254]